Gutiérrez-Escolano A L, Medina F, Racaniello V R, Del Angel R M
Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México City, Mexico.
Virology. 1997 Jan 20;227(2):505-8. doi: 10.1006/viro.1996.8330.
The restricted tissue tropism observed in poliovirus infection is not governed solely by the expression of the poliovirus receptor (PVR) gene, but might be controlled at stages beyond virus entry, such as translation, replication, or assembly. Translation of poliovirus RNA by a cap-independent mechanism requires interactions of the 5'-untranslated region (5'UTR) with cell proteins. To determine whether the patterns of these interacting proteins differ in HeLa cells and permissive and nonpermissive tissues, UV-crosslinking assays using the poliovirus 5'UTR and tissue extracts from PVR transgenic mice were performed. The results indicate a correlation between the presence of a 97-kDa UV-crosslinked protein and permissivity to poliovirus infection. Acquired poliovirus susceptibility in in vitro-cultured kidney cells also correlates with the presence of a 97-kDa crosslinked band. The interaction of the 97-kDa protein from HeLa cells and mouse brain with the poliovirus 5'UTR is stable and specific. Whether the 97-kDa protein plays a role in poliovirus translation and tissue susceptibility remains to be determined.
脊髓灰质炎病毒感染中观察到的组织嗜性受限并非仅由脊髓灰质炎病毒受体（PVR）基因的表达所决定，而是可能在病毒进入后的阶段受到控制，例如翻译、复制或组装。脊髓灰质炎病毒RNA通过不依赖帽结构的机制进行翻译需要5'-非翻译区（5'UTR）与细胞蛋白相互作用。为了确定这些相互作用蛋白的模式在HeLa细胞以及允许和不允许感染的组织中是否不同，使用脊髓灰质炎病毒5'UTR和PVR转基因小鼠的组织提取物进行了紫外线交联试验。结果表明，97 kDa紫外线交联蛋白的存在与对脊髓灰质炎病毒感染的易感性之间存在相关性。体外培养的肾细胞中获得的脊髓灰质炎病毒易感性也与97 kDa交联带的存在相关。HeLa细胞和小鼠脑中97 kDa蛋白与脊髓灰质炎病毒5'UTR的相互作用是稳定且特异的。97 kDa蛋白是否在脊髓灰质炎病毒翻译和组织易感性中起作用还有待确定。
