营养物质对棕色固氮菌中藻酸盐合成的影响及所产藻酸盐的特性
Effect of nutrients on alginate synthesis in Azotobacter vinelandii and characterization of the produced alginate.
作者信息
Sabry S A, Ghanem K M, Sabra W A
机构信息
Department of Botany and Microbiology, Faculty of Science, Alexandria University, Egypt.
出版信息
Microbiologia. 1996 Dec;12(4):593-8.
The role of nutrients on alginate production by Azotobacter vinelandii was studied in batch cultures. The largest amount of bacterial alginate was obtained in presence of: 0.3 g/l MgSO4.7H2O. 0.4 g/l NaCl, 42 mg/l CaCl2.2H2O,.4 mg/l KH2PO4, 16 mg/l K2HPO4, 2.5 mg/l FeSO4.7H2O, 2.9 mg/l H3BO3, 2 mg/l ZnSO4.7H2O, 2 mg/l Na2MoO4.2H2O, 0.3 mg/l CuSO4.5H2O, 0.2 mg/l MnCl2.4H2O. Alginate production was not enhanced by natural additives or inducing agents, except for acetate, which increased alginate yield. The pure alginate contained 0.36% ash and 0.4% protein. It is similar to algal alginate, but it has an extra acetyl group. It contains 69.5% M-M block, 27.5% M-G block and 3% G-G block.
在分批培养中研究了营养物质对棕色固氮菌产藻酸盐的作用。在存在以下物质的情况下可获得最大量的细菌藻酸盐：0.3 g/l七水硫酸镁、0.4 g/l氯化钠、42 mg/l二水氯化钙、0.4 mg/l磷酸二氢钾、16 mg/l磷酸氢二钾、2.5 mg/l七水硫酸亚铁、2.9 mg/l硼酸、2 mg/l七水硫酸锌、2 mg/l二水钼酸钠、0.3 mg/l五水硫酸铜、0.2 mg/l四水氯化锰。除了能提高藻酸盐产量的乙酸盐外，天然添加剂或诱导剂均不能提高藻酸盐产量。纯藻酸盐含有0.36%的灰分和0.4%的蛋白质。它与海藻藻酸盐相似，但有一个额外的乙酰基。它含有69.5%的M-M嵌段、27.5%的M-G嵌段和3%的G-G嵌段。
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