Orian-Rousseau V, Aberdam D, Fontao L, Chevalier L, Meneguzzi G, Kedinger M, Simon-Assmann P
INSERM U. 381, Nice, France.
Dev Dyn. 1996 May;206(1):12-23. doi: 10.1002/(SICI)1097-0177(199605)206:1<12::AID-AJA2>3.0.CO;2-3.
We report the expression pattern of hemidesmosome-associated proteins laminin-5, composed of alpha 3 beta 3 gamma 2 chains, and HD1 in the developing mouse and human intestine, an organ in which variations in structure and function parallel morphogenesis and differentiation. Immunocytochemistry analysis revealed the coexpression of laminin-5 and HD1 at the basal pole of differentiating epithelial cells. Distinct noticeable variations occurring in the location of laminin alpha 3 chain in development of mouse gut were stressed by the reverse transcriptase-polymerase chain reaction data. A peculiar finding was also the location of laminin gamma 2 chain in the intestinal muscle coat. The cellular origin of laminin gamma 2 chain was examined by immunocytochemistry on interspecies hybrid intestines with specific antibodies recognizing mouse antigens. Complementary and sequential production of laminin gamma 2 chain was observed, by epithelial cells as establishment of the basement membrane occurs and by mesenchymal cells in the more differentiated organ. These results support the concept of mesenchymal involvement in deposition of basement membrane molecules, a crucial process for intestinal differentiation. Taken together these data provide the first evidence for the coexpression of hemidesmosome-associated proteins in the gut, a non-stratified tissue.
我们报道了由α3β3γ2链组成的半桥粒相关蛋白层粘连蛋白-5和HD1在发育中的小鼠和人类肠道中的表达模式，肠道是一个结构和功能的变化与形态发生和分化平行的器官。免疫细胞化学分析显示，层粘连蛋白-5和HD1在分化的上皮细胞基极共表达。逆转录聚合酶链反应数据强调了小鼠肠道发育过程中层粘连蛋白α3链位置出现的明显显著变化。一个特殊的发现是层粘连蛋白γ2链在肠肌层中的位置。通过用识别小鼠抗原的特异性抗体对种间杂交肠道进行免疫细胞化学分析，研究了层粘连蛋白γ2链的细胞起源。观察到层粘连蛋白γ2链由上皮细胞在基底膜形成时以及在分化程度更高的器官中的间充质细胞互补性地依次产生。这些结果支持了间充质参与基底膜分子沉积的概念，这是肠道分化的一个关键过程。综合这些数据为半桥粒相关蛋白在肠道（一种非分层组织）中的共表达提供了首个证据。
