Connell G J, Byrne E M, Simpson L
Howard Hughes Medical Institute, University of California, Los Angeles, California 90095-1662, USA.
J Biol Chem. 1997 Feb 14;272(7):4212-8. doi: 10.1074/jbc.272.7.4212.
A primer extension assay was used for the detection of uridine insertions occurring in vitro in synthetic pre-edited cytochrome b mRNA during incubation with a Leishmania tarentolae mitochondrial extract. Two different activities were detected that inserted uridines within the first two editing sites: one that is dependent on the secondary structure of the mRNA but is independent of both exogenous and endogenous guide RNA, and a second that does not put the same structural constraints on the mRNA, but is dependent on the presence of a cognate guide RNA.
采用引物延伸分析来检测在与墨西哥利什曼原虫线粒体提取物孵育期间，合成的预编辑细胞色素b mRNA在体外发生的尿苷插入情况。检测到两种不同的活性，它们在前两个编辑位点内插入尿苷：一种活性依赖于mRNA的二级结构，但不依赖于外源性和内源性引导RNA；另一种活性对mRNA没有相同的结构限制，但依赖于同源引导RNA的存在。
