Czokało M, Silko J, Topczewska E
Department of General and Experimental Pathology, Medical Academy of Białystok.
Rocz Akad Med Bialymst. 1996;41(2):441-51.
The samples of parotid gland saliva, collected from control human subjects and those taken from patients with head and neck cancers were submitted to the assay of protein concentration and kininogenase and amidolytic kallikrein activities. No patients with parotid gland tumours were included. The effect of pilocarpine stimulation on these parameters was studied. It was found that the saliva secreted by parotid gland of the investigated patients contains less protein and lower kininogenase activity in comparison to control subjects. Pilocarpine administration resulted in an increase of protein concentration and a decrease of kallikrein activity both in control and investigated subjects. Radiotherapy did not evoke any significant changes in spontaneously secreted saliva. The radiotherapy resulted in a progressive decrease of protein concentration and kallikrein activity in saliva secreted by pilocarpine stimulated glands. The kallikrein activity per mg of protein contained in spontaneously secreted saliva increased significantly during radiotherapy but it distinctly decreased in the saliva of pilocarpine-treated patients. It allows to conclude that the parotid glands do not lose their ability to synthesize and secrete kallikrein during radiotherapy of head and neck cancers.
收集了来自健康对照者以及头颈癌患者的腮腺唾液样本，进行蛋白质浓度、激肽原酶和酰胺水解性激肽释放酶活性检测。样本中不包括腮腺肿瘤患者。研究了毛果芸香碱刺激对这些参数的影响。结果发现，与对照者相比，所研究患者腮腺分泌的唾液中蛋白质含量较少，激肽原酶活性较低。给予毛果芸香碱后，对照者和研究对象的唾液蛋白质浓度均升高，激肽释放酶活性均降低。放疗对自发分泌的唾液未引起任何显著变化。放疗导致毛果芸香碱刺激腺体分泌的唾液中蛋白质浓度和激肽释放酶活性逐渐降低。放疗期间，自发分泌唾液中每毫克蛋白质所含的激肽释放酶活性显著增加，但在毛果芸香碱治疗患者的唾液中明显降低。由此可以得出结论，在头颈癌放疗期间，腮腺合成和分泌激肽释放酶的能力并未丧失。
