克隆解脂耶氏酵母中酿酒酵母SEC62基因的同源物。

Cloning the Yarrowia lipolytica homologue of the Saccharomyces cerevisiae SEC62 gene.

作者信息

Swennen D, Joyet P, Gaillardin C

机构信息

Laboratoire de Génétique Moléculaire et Cellulaire, INRA, CNRS, INA Paris-Grignon, F-78850 Thiverval Grignon, France.

出版信息

Curr Genet. 1997 Feb;31(2):128-32. doi: 10.1007/s002940050186.

DOI:10.1007/s002940050186

PMID:9021129

Abstract

Yarrowia lipolytica SEC62 cDNA was cloned by functional complementation of a thermo-sensitive sec62 Saccharomyces cerevisiae mutant strain. The Y. lipolytica SEC62 promoter region was amplified by the inverse polymerase chain reaction (PCR). The cDNA codes for a 396 amino-acid protein with two potential trans-membrane domains. Y. lipolytica Sec62p behaves as an integral membrane protein as shown by Western blotting. Y. lipolytica SEC62 cDNA is able to complement a S. cerevisiae sec62 null mutant strain confirming functional conservation, although only 53.6% amino-acid similarity is observed between Y. lipolytica and S. cerevisiae Sec62.

摘要

解脂耶氏酵母SEC62 cDNA通过对温度敏感的酿酒酵母sec62突变菌株进行功能互补克隆得到。解脂耶氏酵母SEC62启动子区域通过反向聚合酶链反应（PCR）扩增。该cDNA编码一个含有两个潜在跨膜结构域的396个氨基酸的蛋白质。蛋白质免疫印迹显示，解脂耶氏酵母Sec62p表现为一种整合膜蛋白。解脂耶氏酵母SEC62 cDNA能够互补酿酒酵母sec62缺失突变菌株，证实了功能保守性，尽管解脂耶氏酵母和酿酒酵母Sec62之间仅观察到53.6%的氨基酸相似性。