Tréton B Y, Le Dall M T, Gaillardin C M
INRA, Centre de Biotechnologies Agro-Industrielles, INA-PG, Thiverval-Grignon, France.
Curr Genet. 1992 Nov;22(5):345-55. doi: 10.1007/BF00352435.
A Yarrowia lipolytica gene library was constructed in vector YRp7 and transformed into a Saccharomyces cerevisiae strain lacking both major acid phosphatase activities. A 2.18 kb genomic sequence restoring the ability to hydrolyze alpha-naphthyl phosphate was isolated. Its sequencing revealed an ORF encoding 358 amino acids without significant homology with any known phosphatase. A putative signal peptide and several possible sites for N-glycosylation were identified. Phosphate-regulated expression of the cloned gene was observed in Y. lipolytica. Disruption data favoured the hypothesis that it might encode a minor phosphatase species.
解脂耶氏酵母基因文库构建于载体YRp7中，并转化到缺乏两种主要酸性磷酸酶活性的酿酒酵母菌株中。分离出一段2.18 kb的基因组序列，该序列恢复了水解α-萘基磷酸的能力。对其测序揭示了一个编码358个氨基酸的开放阅读框，与任何已知磷酸酶均无明显同源性。鉴定出一个推定的信号肽和几个可能的N-糖基化位点。在解脂耶氏酵母中观察到克隆基因的磷酸盐调节表达。破坏数据支持了它可能编码一种次要磷酸酶种类的假说。
