Rao K B, Bhat K V, Totey S M
Embryo Biotechnology Laboratory, National Institute of Immunology, New Delhi, India.
Genet Anal. 1996 Nov;13(5):135-8. doi: 10.1016/s1050-3862(96)00163-5.
The potential use of random amplified polymorphic DNA (RAPD) was evaluated as a source of development of alternative genetic markers for studying variation in buffalo (Bubalus bubalis) and other related species of the Artiodactyla family Bovidae, in order to ascertain genetic relationships and diversities. Fourteen arbitrary primers were used to amplify DNA fragments in four species such as Indian Zebu cattle (Bos indicus), buffalo (Bubalus bubalis), sheep (Ovis aries) and goat (Capra hircus). Clear and distinct RAPD patterns with a higher level of polymorphism was detected between species, while fewer polymorphisms were found within the species. Species were subsequently scored for presence or absence of RAPD fragments and Jaccard's similarity coefficients were calculated to quantify the genetic divergence among the species. Wagner parsimony analysis of the RAPD data for 542 markers resulted in one most parsimonious tree which revealed very low similarity among the four species analysed.
为了确定遗传关系和多样性，评估了随机扩增多态性DNA（RAPD）作为开发替代遗传标记的来源，用于研究水牛（Bubalus bubalis）以及偶蹄目牛科其他相关物种的变异情况。使用14个任意引物对印度瘤牛（Bos indicus）、水牛（Bubalus bubalis）、绵羊（Ovis aries）和山羊（Capra hircus）这四个物种的DNA片段进行扩增。在物种之间检测到具有较高多态性水平的清晰且独特的RAPD模式，而在物种内部发现的多态性较少。随后对物种的RAPD片段的存在与否进行评分，并计算杰卡德相似系数以量化物种之间的遗传差异。对542个标记的RAPD数据进行瓦格纳简约分析，得到一棵最简约的树，该树显示所分析的四个物种之间的相似性非常低。
