Capková J, Pĕknicová J
Department of Biology and Biochemistry of Fertilization, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Mol Reprod Dev. 1997 Feb;46(2):168-75. doi: 10.1002/(SICI)1098-2795(199702)46:2<168::AID-MRD7>3.0.CO;2-O.
The effect of seminal plasma and low molecular weight ACR.3 (17 kDa) protein on boar spermatozoa-porcine oocyte binding was examined. Boar seminal plasma that contains the sperm-adhesive ACR.3 protein was added to spermatozoa prior to their coincubation with oocytes, and the binding capacity of the spermatozoa so treated was compared to that of untreated cells. Similarly, purified ACR.3 protein, that binds to the egg zona pellucida, was added to noncapacitated spermatozoa, and the binding capacity of treated and untreated cells was again evaluated. In the two cases, the treatment of spermatozoa reduced their capacity to bind to the zona pellucida. We propose that the reduction in binding is due to competition for the ACR.3 binding sites on the zona pellucida between the soluble ACR.3 protein and the ACR.3 protein attached to the sperm surface. Furthermore, sperm-ZP binding was examined in the presence of ACR.3 monoclonal antibody, which specifically reacts with ACR.3 protein. Preliminary results show that addition of ACR.3 monoclonal antibody to a suspension of boar spermatozoa prior to their coincubation with oocytes did not markedly change sperm-zona binding in comparison with the control untreated spermatozoa. Our results suggest that ACR.3 protein may mediate the primary sperm-egg zona pellucida binding, and that it is one of the likely candidates for the primary sperm-ZP binding protein.
研究了精浆和低分子量ACR.3（17 kDa）蛋白对公猪精子与猪卵母细胞结合的影响。在精子与卵母细胞共孵育之前，将含有精子黏附性ACR.3蛋白的公猪精浆添加到精子中，并将如此处理后的精子的结合能力与未处理细胞的结合能力进行比较。同样，将与卵透明带结合的纯化ACR.3蛋白添加到未获能的精子中，再次评估处理和未处理细胞的结合能力。在这两种情况下，精子处理均降低了它们与透明带结合的能力。我们认为结合能力的降低是由于可溶性ACR.3蛋白与附着在精子表面的ACR.3蛋白之间竞争透明带上的ACR.3结合位点所致。此外，在存在ACR.3单克隆抗体的情况下检测精子与透明带的结合，该单克隆抗体可与ACR.3蛋白特异性反应。初步结果表明，在公猪精子与卵母细胞共孵育之前，向精子悬液中添加ACR.3单克隆抗体，与未处理的对照精子相比，精子与透明带的结合没有明显变化。我们的结果表明，ACR.3蛋白可能介导精子与卵透明带的初始结合，并且它是精子与透明带初始结合蛋白的可能候选者之一。
