Luhtala M, Lassila O, Toivanen P, Vainio O
Turku Immunology Center, Department of Medical Microbiology, Turku University, Finland.
Eur J Immunol. 1997 Jan;27(1):189-93. doi: 10.1002/eji.1830270128.
In this study we show the inheritance of a CD4+ CD8+ peripheral Tcell population in the H.B15 chicken strain. A large proportion of alphabeta T cells in peripheral blood (20-40%), spleen (10-20%) and intestinal epithelium (5-10%) coexpress CD4 and CD8alpha, but not CD8beta. CD4+ CD8alpha alpha cells are functionally normal T cells, since they proliferate in response to mitogens and signals delivered via the alphabeta T cell receptor as well as via the CD28 co-receptor. These cells induce in vivo a graft versus host-reaction, providing further evidence for their function as CD4+ T cells. The CD4+ CD8alpha alpha T cell population was found in 75% of the first progeny and in 100% of further progenies, demonstrating that coexpression of CD4 and CD8 on peripheral T cells is an inherited phenomenon. In addition, cross-breeding data suggest a dominant Mendelian form of inheritance. The hereditary expression of CD8alpha on peripheral CD4+ T cells in chicken provides a unique model in which to study the regulation of CD4 and CD8 expression.
在本研究中，我们展示了H.B15鸡品系中CD4⁺CD8⁺外周T细胞群体的遗传特性。外周血（20% - 40%）、脾脏（10% - 20%）和肠上皮（5% - 10%）中的大部分αβ T细胞共表达CD4和CD8α，但不表达CD8β。CD4⁺CD8αα细胞是功能正常的T细胞，因为它们能对丝裂原以及通过αβ T细胞受体和CD28共受体传递的信号作出增殖反应。这些细胞在体内可诱导移植物抗宿主反应，为其作为CD4⁺ T细胞的功能提供了进一步证据。在75%的第一代子代和100%的后续子代中都发现了CD4⁺CD8αα T细胞群体，这表明外周T细胞上CD4和CD8的共表达是一种遗传现象。此外，杂交数据表明这是一种显性孟德尔遗传形式。鸡外周CD4⁺ T细胞上CD8α的遗传性表达提供了一个研究CD4和CD8表达调控的独特模型。
