碱性成纤维细胞生长因子及其mRNA在晚期子宫颈癌中的表达

Expression of basic fibroblast growth factor and its mRNA in advanced uterine cervical cancers.

作者信息

Fujimoto J, Ichigo S, Hori M, Hirose R, Sakaguchi H, Tamaya T

机构信息

Department of Obstetrics and Gynecology, Gifu University School of Medicine, Gifu City, Japan.

出版信息

Cancer Lett. 1997 Jan 1;111(1-2):21-6. doi: 10.1016/s0304-3835(96)04485-0.

DOI:10.1016/s0304-3835(96)04485-0

PMID:9022124

Abstract

To know the potential of growth, invasion and metastasis of uterine cervical cancer cells associated with neovascularization, the expression of basic fibroblast growth factor (FGF) and its mRNA in uterine cervical cancers and normal uterine cervices as controls were determined by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction-Southern blot (RT-PCR-SB), respectively. Then, the relations between the expression and the histological grading and clinical staging in cervical cancers were analyzed. The levels of basic FGF and its mRNA were significantly higher in advanced primary uterine cervical cancers, regardless of histological type. Therefore, this status might contribute to the acceleration of growth, invasion, and metastasis with neovascularization in advanced uterine cervical cancers.

摘要

为了解与新生血管形成相关的子宫颈癌细胞的生长、侵袭和转移潜能，分别采用酶联免疫吸附测定法（ELISA）和逆转录-聚合酶链反应- Southern印迹法（RT-PCR-SB）检测子宫颈癌组织及作为对照的正常子宫颈组织中碱性成纤维细胞生长因子（FGF）的表达及其mRNA水平。然后，分析其表达与子宫颈癌组织学分级及临床分期之间的关系。无论组织学类型如何，晚期原发性子宫颈癌中碱性FGF及其mRNA水平均显著升高。因此，这种状态可能有助于晚期子宫颈癌中伴随新生血管形成的生长、侵袭及转移的加速。