Berry D L, Lieber M R, Fischer S M, Slaga T J
Cancer Lett. 1977 Sep;3(3-4):125-32. doi: 10.1016/s0304-3835(77)94943-6.
A high-pressure liquid chromatographic (HPLC) method using a micro-particulate silica column and gradient elution was developed that separated 12-O-tetradecanoylphorbol-13-acetate (TPA) from 20-oxo-TPA; 12-O-tetradecanoylphorbol (TP); 13-O-acetylphorbol (PA), and from the diterpene alcohol, phorbol (P). A series of other phorbol-ester tumor promoters were also separated via HPLC. Spectrophotometric determination at 232 nm allowed detection sensitivities of 0.05 microgram of TPA. When tritiated TPA was applied to mouse skin, the majority of the tritiated product recovered was TPA, indicating only minimal metabolism of TPA and no need for metabolic activation for tumor promotion.
开发了一种使用微颗粒硅胶柱和梯度洗脱的高压液相色谱（HPLC）方法，该方法可将12-O-十四烷酰佛波醇-13-乙酸酯（TPA）与20-氧代-TPA、12-O-十四烷酰佛波醇（TP）、13-O-乙酰佛波醇（PA）以及二萜醇佛波醇（P）分离。一系列其他佛波酯肿瘤促进剂也通过HPLC进行了分离。在232nm处进行分光光度测定，TPA的检测灵敏度可达0.05微克。当将氚标记的TPA应用于小鼠皮肤时，回收的大部分氚标记产物为TPA，这表明TPA的代谢极少，且肿瘤促进不需要代谢激活。
