Bailly C, Denny W A, Waring M J
Institut de Recherches sur le Cancer, INSERM Unité 124, Lille, France.
Anticancer Drug Des. 1996 Dec;11(8):611-24.
To determine the molecular origins of the preferential binding of an antitumour amsacrine-4-carboxamide derivative to GC-rich sequences in DNA, we have used the polymerase chain reaction to synthesize a series of oligodeoxynucleotides in which the position of the purine 2-amino group is varied and then investigated the binding of the drug to normal and modified DNA molecules by means of DNase I footprinting. The results indicate that the 2-amino group of guanine represents an important but not unique element which directs selective binding of amsacrine-4-carboxamides to GC-rich sequences.
为了确定一种抗肿瘤安吖啶 - 4 - 羧酰胺衍生物与DNA中富含GC序列优先结合的分子起源，我们利用聚合酶链反应合成了一系列嘌呤2 - 氨基位置不同的寡脱氧核苷酸，然后通过DNase I足迹法研究了该药物与正常及修饰DNA分子的结合情况。结果表明，鸟嘌呤的2 - 氨基是指导安吖啶 - 4 - 羧酰胺选择性结合富含GC序列的重要但非唯一的元素。
