Michelangeli V P, Heyma P, Colman P G, Ebeling P R
Department of Diabetes and Endocrinology, Royal Melbourne Hospital, Parkville, Victoria, Australia.
Ann Clin Biochem. 1997 Jan;34 ( Pt 1):97-103. doi: 10.1177/000456329703400115.
The Immulite parathyroid hormone (PTH) immunochemiluminometric assay (ICMA) was evaluated by measuring serum PTH concentrations in 134 volunteers and 25 patients with various diseases affecting bone and mineral metabolism and comparing with those measured by the Nichols Allegro immunoradiometric (IRMA) assay. Although the assays were highly correlated (r = 0.99. P < 0.0001), there were discrepancies at lower PTH concentrations which may be explained by the lower limit of detection of the ICMA assay. The anti-coagulants EDTA and heparin appeared to have significant effects on PTH measurement particularly at lower concentrations. Sera assayed by ICMA diluted out more uniformly than in the IRMA and there was no cross reactivity with parathyroid hormone-related peptide in either the IRMA or ICMA. Delays of up to 8 h in specimen handling did not appear to affect PTH measurements. The enhanced sensitivity of the Immulite ICMA intact PTH assay confers an advantage in the diagnosis of both hyper- and hypocalcaemia, while the shorter incubation times, automation and lack of radioactivity are important practical advantages.
通过检测134名志愿者以及25名患有影响骨和矿物质代谢的各种疾病的患者的血清甲状旁腺激素（PTH）浓度，并与采用Nichols Allegro免疫放射分析（IRMA）法测得的结果进行比较，对Immulite甲状旁腺激素免疫化学发光分析（ICMA）法进行了评估。尽管两种检测方法高度相关（r = 0.99，P < 0.0001），但在较低的PTH浓度下存在差异，这可能是由ICMA检测法的检测下限较低所致。抗凝剂乙二胺四乙酸（EDTA）和肝素似乎对PTH测量有显著影响，尤其是在较低浓度时。与IRMA法相比，ICMA法检测的血清稀释更均匀，并且在IRMA法或ICMA法中均未与甲状旁腺激素相关肽发生交叉反应。样本处理延迟长达8小时似乎并未影响PTH测量。Immulite ICMA完整PTH检测法灵敏度的提高在高钙血症和低钙血症的诊断中具有优势，而较短的孵育时间、自动化以及无放射性则是重要的实际优势。
