MacNeil R L, Berry J, Strayhorn C, Somerman M J
Department of Periodontics/Prevention/Geriatrics, University of Michigan, Ann Arbor 48109-1078, USA.
Arch Oral Biol. 1996 Aug-Sep;41(8-9):827-35. doi: 10.1016/s0003-9969(96)00051-9.
Adhesion molecules are considered to have an active role in controlling cell differentiation, although the mechanisms involved have yet to be determined. The developing tooth provides an excellent model to use for determining the factors/processes regulating cell differentiation. The studies presented here focused specifically on the timed and spatial expression of a bone-associated adhesion molecule, bone sialoprotein, during tooth root development. Mandibular tissues in the first molar region of CD-1 mice, at sequential stages of development, were analysed by in situ hybridization. The results demonstrate distinct expression of bone sialoprotein in surrounding bone at early stages of tooth development. At stages of active cementogenesis, bone sialoprotein transcripts were specific to cells lining the root surface, with limited expression in the surrounding connective tissue (periodontal ligament) region. The strong expression of bone sialoprotein, a mineral-specific protein having the capacity to act as a nucleator of hydroxyapatite in vitro, by cells lining the root surface at early stages of cementogenesis suggests that this molecule is operative in the cell/matrix events that accompany cementum formation.
黏附分子被认为在控制细胞分化中发挥着积极作用，尽管其中涉及的机制尚未确定。发育中的牙齿为确定调节细胞分化的因素/过程提供了一个绝佳的模型。本文所呈现的研究特别聚焦于一种与骨相关的黏附分子——骨唾液蛋白在牙根发育过程中的时空表达。通过原位杂交分析了处于连续发育阶段的CD - 1小鼠第一磨牙区域的下颌组织。结果表明，在牙齿发育早期，骨唾液蛋白在周围骨中有明显表达。在活跃的牙骨质形成阶段，骨唾液蛋白转录本特异性地存在于根表面的细胞中，在周围结缔组织（牙周韧带）区域表达有限。在牙骨质形成早期，根表面的细胞强烈表达骨唾液蛋白，这种矿物质特异性蛋白在体外具有作为羟基磷灰石成核剂的能力，这表明该分子在伴随牙骨质形成的细胞/基质事件中起作用。
