Mirchamsy H, Neway T, Hamedi M, Pilet C
Razi State Serum and Vaccine Institute, Tehran, Islamic Republic of Iran.
Comp Immunol Microbiol Infect Dis. 1997 Jan;20(1):13-20. doi: 10.1016/s0147-9571(96)00030-6.
Experiments were carried out to examine the adjuvanticity of polar glycopeptidolipids of Mycobacterium chelonae (pGPL-Mc) or the London rocket seed (LRS) when combined with diphtheria and tetanus toxoids in an oral immunization of the African green monkey. The results showed that none of the monkeys receiving diphtheria and tetanus toxoids combined with 25 mg/kg of pGPL-Mc showed an increase in the the level of diphtheria antitoxin (DA) on the third and sixth weeks following the first and the second immunizations. One monkey from this group responded with increased seroneutralizing antibodies 3 weeks after the third feeding. On the other hand, one monkey, 3 weeks after the first immunization, and three monkeys, 3 weeks after the second and third oral vaccinations, showed an increase in specific anti-diphtheria antibody responses when the toxoids were combined with 25 mg/kg of LRS. The anti-diphtheria antitoxin responses of monkeys receiving diphtheria and tetanus toxoids combined with 50 mg/kg of pGPL-Mc or 50 mg/kg of LRS were significantly enhanced compared to the groups administered 25 mg/kg of the two adjuvants. The increase was observed in four out of five pGPL-Mc administered and in three out of five LRS-receiving monkeys. The results show that pGPL-Mc induced the highest titres of anti-diphtheria antitoxin compared to LRS, whereas the level of anti-diphtheria antitoxin titre of the two monkeys receiving the toxoids alone was less than 0.1 i.u./ml of serum throughout the experiment. According to the statistical analyses, no significant differences were recorded between the diphtheria antitoxin responses of monkeys following the first, second or third administration of LRS-adjuvated diphtheria and tetanus toxoids. However, a significant difference (P < or = 0.05) was observed in the diphtheria antitoxin response between the first and the second immunization of monkeys administered with toxoids adjuvated with 50 mg/kg of pGPL-Mc. The tetanus antitoxin responses of all monkeys were less than 0.1 i.u. of antitoxin per millilitre of serum throughout the study, which is considered not to be protective. However, we have recorded an anti-tetanus antitoxin titre of more than 0.2 i.u./ml of serum in one monkey that received diphtheria and tetanus toxoids combined with 50 mg/kg of pGPL-Mc.
开展了实验，以检测龟分枝杆菌的极性糖肽脂（pGPL-Mc）或伦敦火箭籽（LRS）与白喉和破伤风类毒素联合用于非洲绿猴口服免疫时的佐剂活性。结果显示，在首次和第二次免疫后的第三周和第六周，接受白喉和破伤风类毒素与25mg/kg pGPL-Mc联合免疫的猴子中，没有一只的白喉抗毒素（DA）水平升高。该组中有一只猴子在第三次喂食后3周出现血清中和抗体增加。另一方面，一只猴子在首次免疫后3周，以及三只猴子在第二次和第三次口服疫苗接种后3周，当类毒素与25mg/kg LRS联合时，显示出特异性抗白喉抗体反应增加。与给予25mg/kg这两种佐剂的组相比，接受白喉和破伤风类毒素与50mg/kg pGPL-Mc或50mg/kg LRS联合免疫的猴子的抗白喉抗毒素反应显著增强。在给予50mg/kg pGPL-Mc的五只猴子中有四只出现增加，在接受50mg/kg LRS的五只猴子中有三只出现增加。结果表明，与LRS相比，pGPL-Mc诱导的抗白喉抗毒素滴度最高，而在整个实验过程中，仅接受类毒素的两只猴子的抗白喉抗毒素滴度水平低于0.1 i.u./ml血清。根据统计分析，在接受LRS佐剂的白喉和破伤风类毒素的猴子的首次、第二次或第三次给药后的白喉抗毒素反应之间没有记录到显著差异。然而，在接受50mg/kg pGPL-Mc佐剂的类毒素的猴子的首次和第二次免疫之间观察到白喉抗毒素反应有显著差异（P≤0.05）。在整个研究过程中，所有猴子的破伤风抗毒素反应均低于每毫升血清0.1 i.u.抗毒素，这被认为没有保护作用。然而，我们在一只接受白喉和破伤风类毒素与50mg/kg pGPL-Mc联合免疫的猴子中记录到血清抗破伤风抗毒素滴度超过0.2 i.u./ml。
