Chaung W, Mi L J, Boorstein R J
Department of Pathology, New York University School of Medicine, Sackler Institute of Graduate Biomedical Sciences, New York, NY 10016, USA.
Nucleic Acids Res. 1997 Mar 1;25(5):992-4. doi: 10.1093/nar/25.5.992.
Chinese hamster lung fibroblast V79 cells have been widely used in studies of DNA damage and DNA repair. Since the p53 gene is involved in normal responses to DNA damage, we have analyzed the molecular genetics and functional status of p53 in V79 cells and primary Chinese hamster embryonic fibroblast (CHEF) cells. The coding product of the p53 gene in CHEF cells was 76 and 75% homologous to human and mouse p53 respectively, and was 95% homologous to the Syrian hamster cells. The V79 p53 sequence contained two point mutations located within a presumed DNA binding domain, as compared with the CHEF cells. Additional immunocytochemical and molecular studies confirmed that the p53 protein in V79 cells was mutated and nonfunctional. Our results indicate that caution should be used in interpreting studies of DNA damage, DNA repair and apoptosis in V79 cells.
中国仓鼠肺成纤维细胞V79已广泛应用于DNA损伤和DNA修复的研究。由于p53基因参与对DNA损伤的正常反应，我们分析了V79细胞和原代中国仓鼠胚胎成纤维细胞（CHEF）中p53的分子遗传学和功能状态。CHEF细胞中p53基因的编码产物分别与人及小鼠的p53有76%和75%的同源性，与叙利亚仓鼠细胞有95%的同源性。与CHEF细胞相比，V79 p53序列在一个假定的DNA结合域内有两个点突变。额外的免疫细胞化学和分子研究证实，V79细胞中的p53蛋白发生了突变且无功能。我们的结果表明，在解释V79细胞中DNA损伤、DNA修复和细胞凋亡的研究时应谨慎。
