Kim C S, Lee C H, Shin J S, Chung Y S, Hyung N I
Department of Horticultural Science, Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea.
Nucleic Acids Res. 1997 Mar 1;25(5):1085-6. doi: 10.1093/nar/25.5.1085.
Because DNA degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality DNA from plants containing a high content of polyphenolics has been a difficult problem. We demonstrate an easy extraction process by modifying several existing ones. Using this process we have found it possible to isolate DNAs from four fruit trees, grape (Vitis spp.), apple (Malus spp.), pear (Pyrus spp.) and persimmon (Diospyros spp.) and four species of conifer, Pinus densiflora, Pinus koraiensis,Taxus cuspidata and Juniperus chinensis within a few hours. Compared with the existing method, we have isolated high quality intact DNAs (260/280 = 1.8-2.0) routinely yielding 250-500 ng/microl (total 7.5-15 microg DNA from four to five tissue discs).
由于DNA降解是由酚类萜类等次生植物产物介导的，因此从富含多酚的植物中分离高质量DNA一直是个难题。我们通过改进几种现有方法，展示了一种简便的提取过程。利用该过程，我们发现在数小时内就能从四种果树（葡萄（葡萄属）、苹果（苹果属）、梨（梨属）和柿子（柿属））以及四种针叶树（赤松、红松、东北红豆杉和圆柏）中分离出DNA。与现有方法相比，我们常规分离出了高质量的完整DNA（260/280 = 1.8 - 2.0），每微升可产生250 - 500纳克（从四到五个组织切片中总共可获得7.5 - 15微克DNA）。
