一种使用聚乙烯吡咯烷酮（PVP）从果树和针叶树中分离高质量基因组DNA的简单快速方法。
A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP.
作者信息
Kim C S, Lee C H, Shin J S, Chung Y S, Hyung N I
机构信息
Department of Horticultural Science, Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea.
出版信息
Nucleic Acids Res. 1997 Mar 1;25(5):1085-6. doi: 10.1093/nar/25.5.1085.
Abstract
Because DNA degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality DNA from plants containing a high content of polyphenolics has been a difficult problem. We demonstrate an easy extraction process by modifying several existing ones. Using this process we have found it possible to isolate DNAs from four fruit trees, grape (Vitis spp.), apple (Malus spp.), pear (Pyrus spp.) and persimmon (Diospyros spp.) and four species of conifer, Pinus densiflora, Pinus koraiensis,Taxus cuspidata and Juniperus chinensis within a few hours. Compared with the existing method, we have isolated high quality intact DNAs (260/280 = 1.8-2.0) routinely yielding 250-500 ng/microl (total 7.5-15 microg DNA from four to five tissue discs).
摘要
由于DNA降解是由酚类萜类等次生植物产物介导的，因此从富含多酚的植物中分离高质量DNA一直是个难题。我们通过改进几种现有方法，展示了一种简便的提取过程。利用该过程，我们发现在数小时内就能从四种果树（葡萄（葡萄属）、苹果（苹果属）、梨（梨属）和柿子（柿属））以及四种针叶树（赤松、红松、东北红豆杉和圆柏）中分离出DNA。与现有方法相比，我们常规分离出了高质量的完整DNA（260/280 = 1.8 - 2.0），每微升可产生250 - 500纳克（从四到五个组织切片中总共可获得7.5 - 15微克DNA）。
相似文献
1
A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP.一种使用聚乙烯吡咯烷酮（PVP）从果树和针叶树中分离高质量基因组DNA的简单快速方法。
Nucleic Acids Res. 1997 Mar 1;25(5):1085-6. doi: 10.1093/nar/25.5.1085.
2
[Isolation of high-quality genomic DNA from plants].[从植物中分离高质量基因组DNA]
Hunan Yi Ke Da Xue Xue Bao. 2001 Apr 28;26(2):178-80.
3
[An efficient macro-method of genomic DNA isolation from Actinidia chinensis leaves].[一种从中华猕猴桃叶片中高效提取基因组DNA的宏观方法]
Yi Chuan. 2004 Jul;26(4):529-31.
4
Isolation of genomic DNA suitable for community analysis from mature trees adapted to arid environment.从适应干旱环境的成熟树木中分离适合群落分析的基因组 DNA。
Gene. 2011 Nov 10;487(2):156-9. doi: 10.1016/j.gene.2011.06.029. Epub 2011 Jul 5.
5
Fruit Characteristics of Transgenic pear (Pyrus communis L.) Trees During Long-Term Field Trials.转基因梨（Pyrus communis L.）树在长期田间试验中的果实特性。
Plant Foods Hum Nutr. 2023 Jun;78(2):445-451. doi: 10.1007/s11130-023-01076-4. Epub 2023 Jun 24.
6
Rapid and simple methodology for isolation of high quality genomic DNA from coniferous tissues (Taxus baccata).从松柏类组织（欧洲红豆杉）中快速简单地提取高质量基因组 DNA 的方法。
Mol Biol Rep. 2010 Feb;37(2):833-7. doi: 10.1007/s11033-009-9634-z. Epub 2009 Aug 2.
7
DNA from processed and unprocessed wood: factors influencing the isolation success.来自加工木材和未加工木材的DNA：影响分离成功的因素。
Forensic Sci Int Genet. 2009 Jun;3(3):185-92. doi: 10.1016/j.fsigen.2009.01.002. Epub 2009 Feb 4.
8
MicroRNAs in fruit trees: discovery, diversity and future research directions.果树中的微小RNA：发现、多样性及未来研究方向
Plant Biol (Stuttg). 2014 Sep;16(5):856-65. doi: 10.1111/plb.12153. Epub 2014 Feb 19.
9
Recent advances in the cryopreservation of shoot-derived germplasm of economically important fruit trees of Actinidia, Diospyros, Malus, Olea, Prunus, Pyrus and Vitis.近年来，在猕猴桃、柿、苹果、油橄榄、李、梨和葡萄等经济重要果树的茎尖衍生种质的低温保存方面取得了进展。
Biotechnol Adv. 2013 Mar-Apr;31(2):175-85. doi: 10.1016/j.biotechadv.2012.09.004. Epub 2012 Sep 27.
10
An optimized mini-preparation method to obtain high-quality genomic DNA from mature leaves of sunflower.一种从向日葵成熟叶片中获取高质量基因组DNA的优化微量制备方法。
Genet Mol Res. 2007 Oct 5;6(4):1064-71.
引用本文的文献
1
Assessing the effect of sample storage time on viral detection using a rapid and cost-effective CTAB-based extraction method.使用基于十六烷基三甲基溴化铵的快速且经济高效的提取方法评估样本储存时间对病毒检测的影响。
Plant Methods. 2024 May 8;20(1):64. doi: 10.1186/s13007-024-01175-6.
2
Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize.转基因玉米核酸检测技术研究进展。
Int J Mol Sci. 2023 Jul 31;24(15):12247. doi: 10.3390/ijms241512247.
3
What is the "modified" CTAB protocol? Characterizing modifications to the CTAB DNA extraction protocol.什么是“改良的”十六烷基三甲基溴化铵（CTAB）方案？对CTAB DNA提取方案的改良进行特征描述。
Appl Plant Sci. 2023 Jun 2;11(3):e11517. doi: 10.1002/aps3.11517. eCollection 2023 May-Jun.
4
A Fast and Cost-Effective Genotyping Method for CRISPR-Cas9-Generated Mutant Rice Lines.一种用于CRISPR-Cas9基因编辑水稻突变体系的快速且经济高效的基因分型方法。
Plants (Basel). 2023 May 31;12(11):2189. doi: 10.3390/plants12112189.
5
High Prevalence of Clonal Reproduction and Low Genetic Diversity in , a Federally Threatened Florida-Endemic Mint.联邦政府列为受威胁物种的佛罗里达特有薄荷中克隆繁殖的高发生率及低遗传多样性
Plants (Basel). 2023 Feb 17;12(4):919. doi: 10.3390/plants12040919.
6
Genome-wide analysis-based single nucleotide polymorphism marker sets to identify diverse genotypes in cabbage cultivars (Brassica oleracea var. capitata).基于全基因组分析的单核苷酸多态性标记集，用于鉴定不同甘蓝品种（芸薹属芸薹种白菜亚种）的基因型。
Sci Rep. 2022 Nov 21;12(1):20030. doi: 10.1038/s41598-022-24477-y.
7
Cone-setting in spruce is regulated by conserved elements of the age-dependent flowering pathway.云杉中的球果形成受与年龄相关的开花途径的保守元件调控。
New Phytol. 2022 Dec;236(5):1951-1963. doi: 10.1111/nph.18449. Epub 2022 Sep 16.
8
Molecular Characterization and Phylogenetic Analysis of MADS-Box Gene Associated with Stenospermocarpic Seedlessness in Muscadine Grapes.与欧亚葡萄无核结实相关的 MADS-box 基因的分子特征和系统进化分析。
Genes (Basel). 2021 Feb 5;12(2):232. doi: 10.3390/genes12020232.
9
Functional Analysis Reveals the Regulatory Role of Encoding Tonoplast Sugar Transporter in Sugar Accumulation of Peach Fruit.功能分析揭示编码液泡膜糖转运体在桃果实糖积累中的调控作用。
Int J Mol Sci. 2020 Feb 7;21(3):1112. doi: 10.3390/ijms21031112.
10
Reproducible genomic DNA preparation from diverse crop species for molecular genetic applications.从多种作物物种中制备可重复的基因组DNA用于分子遗传学应用。
Plant Methods. 2017 Dec 2;13:106. doi: 10.1186/s13007-017-0255-6. eCollection 2017.
本文引用的文献
1
A simple method of preparing plant samples for PCR.一种制备用于聚合酶链反应的植物样本的简单方法。
Nucleic Acids Res. 1993 Aug 25;21(17):4153-4. doi: 10.1093/nar/21.17.4153.
2
Midiprep method for isolation of DNA from plants with a high content of polyphenolics.从富含多酚的植物中分离DNA的碱裂解法。 （注：这里原文的Midiprep method可能有误，推测正确的是Midi-prep method即碱裂解法，按照碱裂解法翻译了，若原文无误请根据正确的Midiprep method准确翻译）
Nucleic Acids Res. 1993 Jul 11;21(14):3328. doi: 10.1093/nar/21.14.3328.
3
A rapid one-tube genomic DNA extraction process for PCR and RAPD analyses.一种用于PCR和RAPD分析的快速单管基因组DNA提取方法。
Nucleic Acids Res. 1995 Jul 11;23(13):2569-70. doi: 10.1093/nar/23.13.2569-a.
4
An efficient method for isolation of RNA and DNA from plants containing polyphenolics.一种从含多酚类物质的植物中分离RNA和DNA的有效方法。
Nucleic Acids Res. 1992 May 11;20(9):2381. doi: 10.1093/nar/20.9.2381.
Zotero 插件