Blumenthal A B, Clark E J
Cell. 1977 Sep;12(1):183-9. doi: 10.1016/0092-8674(77)90196-9.
The size of DNA replication intermediates from Drosophila cells pulse-labeled with 3H-thymidine for 30-120 sec was determined by electrophoresis in formamide-polyacrylamide gels. Replication intermediates were formed in three discrete size classes, with median lengths of 61, 125 and 240 nucleotides. Replication intermediates in the 125 nucleotide size class occurred most frequently. Two of the three size classes may contain discrete species of replication intermediates about 90-400 nucleotides long. The data also suggested that some larger replication intermediates accumulate in pulse-labeled cells. We concluded that 61 nucleotide molecules give rise to 125 and 240 nucleotide molecules, which then form high molecular weight DNA. Mechanisms for forming these replication intermediates are discussed.
通过在甲酰胺-聚丙烯酰胺凝胶中进行电泳，测定了用³H-胸腺嘧啶脉冲标记30 - 120秒的果蝇细胞中DNA复制中间体的大小。复制中间体形成了三个离散的大小类别，中位数长度分别为61、125和240个核苷酸。125个核苷酸大小类别的复制中间体出现得最为频繁。三个大小类别中的两个可能包含长度约为90 - 400个核苷酸的离散复制中间体种类。数据还表明，一些较大的复制中间体在脉冲标记的细胞中积累。我们得出结论，61个核苷酸的分子产生125和240个核苷酸的分子，然后形成高分子量DNA。文中讨论了形成这些复制中间体的机制。
