Carrillo R, Candia A, Rodriguez-Peralto J L, Caz V
Department of Pathology, Hospital Universitario Ramón y Cajal, Madrid,Spain.
Hum Pathol. 1997 Feb;28(2):160-5. doi: 10.1016/s0046-8177(97)90100-3.
The DNA content and proliferative index of 61 gastrointestinal stromal tumors (GIST) were measured by image analysis and correlated with the lesion's clinicopathological features and patient's survival. DNA analysis was performed on cytospin single-cell preparations obtained from the paraffin-embedded tissue blocks. MIB-1 was the proliferation marker used on paraffin sections. DNA aneuploidy was detected in 12 tumors (18%), and high MIB-1 index (>22%) in 12 lesions (18%). DNA aneuploidy and high MIB-1 index statistically correlated with high mitotic rate (> or = 5 x 10 high-power field [HPF]) (P < .001) and with the presence of necrosis (P < .05). The patient's survival was significantly correlated with DNA ploidy (P < .01), MIB-1 index (P < .00001), mitotic rate (P < .00001), presence of necrosis (P < .0001), and size of the tumor (P < .01). Multivariate regression analysis showed that only MIB-1 index was an independent parameter in predicting the clinical outcome for patients with GIST. The mitotic rate was the only other independent prognostic factor when MIB-1 index was not allowed to enter the model.
通过图像分析测量61例胃肠道间质瘤（GIST）的DNA含量和增殖指数，并将其与病变的临床病理特征及患者生存率相关联。对从石蜡包埋组织块中获得的细胞涂片单细胞制剂进行DNA分析。MIB-1是用于石蜡切片的增殖标志物。在12个肿瘤（18%）中检测到DNA非整倍体，在12个病变（18%）中检测到高MIB-1指数（>22%）。DNA非整倍体和高MIB-1指数与高有丝分裂率（≥5×10高倍视野[HPF]）（P<.001）及坏死的存在（P<.05）在统计学上相关。患者生存率与DNA倍体（P<.01）、MIB-1指数（P<.00001）、有丝分裂率（P<.00001）、坏死的存在（P<.0001）及肿瘤大小（P<.01）显著相关。多因素回归分析显示，只有MIB-1指数是预测GIST患者临床结局的独立参数。当不允许MIB-1指数进入模型时，有丝分裂率是唯一的其他独立预后因素。
