Kuwabara H, Tanaka S, Sakamoto H, Oryu M, Uda H
Department of Pathology, Kagawa Medical University, Japan.
Kobe J Med Sci. 1996 Aug;42(4):233-41.
We investigated the effects on leukocyte adhesion to cultured human dermal microvascular endothelial (DMvE) cell by antibody mediated ligation of platelet/endothelial cell adhesion molecule-1 (PECAM-1) on neutrophils. Preincubation of neutrophils with anti-PECAM-1 antibody markedly enhanced their adhesion to DMvE cells, while the adhesion-increasing action was cancelled out by anti-CR3 or anti-LFA-1 beta (CD18) antibody. These findings suggest that ligation of PECAM-1 on neutrophils increases their adhesion to cultured DMvE cells depending on the beta 2 integrin family, and PECAM-1 may have a crucial role in dermal inflammation.
我们研究了通过抗体介导的中性粒细胞上血小板/内皮细胞黏附分子-1（PECAM-1）的连接对白细胞黏附于培养的人真皮微血管内皮（DMvE）细胞的影响。用抗PECAM-1抗体预孵育中性粒细胞可显著增强其对DMvE细胞的黏附，而抗CR3或抗LFA-1β（CD18）抗体可消除这种黏附增加作用。这些发现表明，中性粒细胞上PECAM-1的连接依赖于β2整合素家族增加其对培养的DMvE细胞的黏附，并且PECAM-1可能在皮肤炎症中起关键作用。
