Holzinger C, Zuckermann A, Reinwald C, Imhof M, Schöllhammer A, Kramer G, Wolner E, Steiner G
Dept. of Cardiac Surgery, aö Kh St. Pölten, Austria.
Immunol Lett. 1996 Nov;53(2-3):63-7. doi: 10.1016/s0165-2478(96)02595-3.
Numerous studies have dealt with occurrence of dendritic cells in various nonlymphoid organs such as kidney, liver or heart, whereas lymphocyte patterns in these organs have not been analyzed in detail. In the present study, leukocytes were quantified as cells/mm2 in the perivascular, interstitial and parenchymal tissue sections of normal heart. We measured an overall mean leukocyte count in normal heart tissue of 17.0 +/- 2.7 CD45+ leukocytes/mm2, 9.1 +/- 1.8 thereof being CD4+ T-helper cells (Th). By comparison, CD8+ T-cytotoxic/suppressor cells (Ts) and CD14+ macrophages each accounted for only approximately 2.5 cells/mm2, and CD20+ B cells for only 1.3 cells/mm2. These T cells were further characterized as either CD45RA+ naive T cells or as CD45RO+ memory T cells. Segmentation of the tissue as defined in Section 2 yielded an ascending number of CD45RO+ memory T cells from perivascular (0.4 +/- 0.2 cells/mm2) through parenchymal (12.8 +/- 3.0 cells/mm2) to interstitial (21.0 +/- 5.3/mm2). By contrast, the number of CD45RA+ and Leu-8+ cells decreased from perivascular to parenchymal. Peripheral T cells showed a reverse pattern of CD45RA/CD45RO antigen expression. Only approximately 3% of T cells expressed activation markers IL-2R and IL7R. Our data demonstrate that the majority of T cells in normal heart tissue are resting memory tissue T cells and are not contaminating T cells from the peripheral blood. The increase in CD45RO+ cells from perivascular to parenchymal with a corresponding decrease in CD45RO+ and Leu-8+ heart-tissue T cells argues in favor of T-cell traffic in normal heart tissue.
许多研究都涉及树突状细胞在各种非淋巴器官（如肾脏、肝脏或心脏）中的出现情况，而这些器官中的淋巴细胞模式尚未得到详细分析。在本研究中，白细胞在正常心脏的血管周围、间质和实质组织切片中以每平方毫米细胞数进行定量。我们测量了正常心脏组织中白细胞的总体平均计数为每平方毫米17.0±2.7个CD45 +白细胞，其中9.1±1.8个为CD4 +辅助性T细胞（Th）。相比之下，CD8 +细胞毒性/抑制性T细胞（Ts）和CD14 +巨噬细胞各自仅占约每平方毫米2.5个细胞，而CD20 + B细胞仅占每平方毫米1.3个细胞。这些T细胞进一步被表征为CD45RA +初始T细胞或CD45RO +记忆T细胞。如第2节所定义的组织分割产生了从血管周围（每平方毫米0.4±0.2个细胞）到实质（每平方毫米12.8±3.0个细胞）再到间质（每平方毫米21.0±5.3个细胞）的CD45RO +记忆T细胞数量增加。相比之下，CD45RA +和Leu - 8 +细胞的数量从血管周围到实质减少。外周T细胞显示出CD45RA/CD45RO抗原表达的相反模式。只有约3%的T细胞表达激活标志物IL - 2R和IL7R。我们的数据表明，正常心脏组织中的大多数T细胞是静止的记忆组织T细胞，而不是来自外周血的污染T细胞。从血管周围到实质的CD45RO +细胞增加，同时心脏组织中CD45RA +和Leu - 8 + T细胞相应减少，这支持了正常心脏组织中的T细胞迁移。
