Morphometric analysis of the human vestibular nuclei.

BACKGROUND

Cytoarchitectural investigations of the vestibular nuclei have been undertaken in different species of mammals. These data provide a description of the general architecture of the nuclei but limited information about quantitative characteristics of their cell population. We have recently obtained data about the morphometric parameters of the vestibular nuclei neurons in some species. The application of quantitative image analysis techniques to the research of the cellular morphology in the vestibular area of humans might provide basic information to compare with data from animal studies, taking into account the observed correlation between physiological and morphological properties of vestibular neurons.

METHODS

The characteristics of the major vestibular nuclei in humans have been studied with light microscopic techniques in serially cut sections. Camera lucida drawings of the vestibular nuclei and their neurons were made and subjected to computerized image analysis. For each vestibular nucleus, information was obtained about topography, morphological characteristics (i.e., location, volume, and length), and the number and morphometric parameters of their neurons (cross-sectional areas, maximum and minimum diameters). Morphometric data about cell parameters were statistically analyzed by comparing the populations within different parts of each nucleus and from different nuclei.

RESULTS

Among the vestibular nuclei, the medial, which is the largest, has the greatest number of neurons, and the interstitial, the least. The lateral and interstitial nuclei contain the largest cells, and the descending nucleus has the smallest cells. The superior nucleus contains cells of intermediate size. The size of cells decreases in a rostrocaudal direction in the medial, lateral, and descending nuclei, the opposite trend being observed in the superior nucleus. Within the superior and medial nuclei, there are discrete areas with cells with distinctive characteristics.

CONCLUSIONS

These results suggest that, just as most of the anatomical characteristics of the second-order neurons found in animals have been preserved in humans, so the physiological mechanisms observed in the vestibular system of animals should apply to humans.