Tarasow T M, Tinnermeier D, Zyzniewski C
NeXstar Pharmaceuticals Inc., Boulder, Colorado 80301, USA.
Bioconjug Chem. 1997 Jan-Feb;8(1):89-93. doi: 10.1021/bc960082+.
Electrospray mass spectrometry (ESMS) was used to characterize a number of differently functionalized polyethylene glycol-oligodeoxyribonucleotide conjugates. Sample preparation was found to be crucial to obtaining quality data. The resolution and precision of ESMS allowed for the identification of individual conjugates differing in MW by a single ethylene glycol unit with an accuracy of < or = 1 (0.02% of the molecular weight). In addition, ESMS was shown to be valuable in identifying chromatographically unresolvable components of a derivatized polyethylene glycol-oligonucleotide conjugate mixture.
电喷雾质谱法（ESMS）被用于表征多种不同功能化的聚乙二醇 - 寡脱氧核糖核苷酸共轭物。发现样品制备对于获得高质量数据至关重要。ESMS的分辨率和精度使得能够鉴定分子量相差一个乙二醇单元的单个共轭物，其准确度小于或等于1（分子量的0.02%）。此外，ESMS在鉴定衍生化的聚乙二醇 - 寡核苷酸共轭物混合物中色谱无法分离的组分方面显示出其价值。
