Grimes J E, Arizmendi F, Carter C N, Sneed L
Department of Microbiology, Texas Veterinary Medical Diagnostic Laboratory, College Station 77841-3040, USA.
J Vet Diagn Invest. 1996 Jan;8(1):38-44. doi: 10.1177/104063879600800107.
A 2 x 2 contingency table was constructed to demonstrate the relationships between detectable chlamydial antibody activity and clinical health status of tested birds. The table revealed that 65.5% of clinically ill birds were antibody positive by elementary body agglutination (EBA) (> or = 10 titers) and 59.0% were antibody positive by latex agglutination (LA). Thus, EBA was slightly more sensitive than LA in detecting antibody activity. Of the clinically normal birds, 96.7% were antibody negative (< 10 titers) by EBA and 98.3% were antibody negative by LA. Individual serum or plasma samples from a group of mixed types of psittacine birds and cockatiels were tested as a separate group, and relationships between EBA-detectable antibody activity and health status were obtained from a 2 x 2 contingency table. Sixty-six percent of birds clinically ill with signs of chlamydiosis in the mixed-type group were antibody positive, whereas only 32.3% of clinically ill cockatiels were antibody positive. Statistical analysis of the contingency table using a chi-square test demonstrated that the EBA test differentiates between individual birds on the basis of health status (P < 0.001). When testing paired serum or plasma samples by EBA, LA, and direct complement fixation (DCF), the highest percentage of significant (> or =4-fold change) titer decreases was detected by LA, and the highest percentage of significant titer increases was detected by DCF. Examples of EBA, LA, and DCF titers in paired and multiple serum or plasma samples are presented to show the variety of responses that can occur. Results reflected variations seen in individual testing of birds with titer variability seen in the first sample tested. Additional types of testing believed necessary for confirming or ruling out an infectious process in birds are outlined. The current interpretations of serologic results are given.
构建了一个2×2列联表来展示检测到的衣原体抗体活性与受试鸟类临床健康状况之间的关系。该表显示，65.5%的临床患病鸟类通过原体凝集试验（EBA）（滴度≥10）抗体呈阳性，59.0%通过乳胶凝集试验（LA）抗体呈阳性。因此，在检测抗体活性方面，EBA比LA稍敏感。在临床正常的鸟类中，96.7%通过EBA抗体呈阴性（滴度<10），98.3%通过LA抗体呈阴性。一组混合类型的鹦鹉和鸡尾鹦鹉的个体血清或血浆样本作为一个单独的组进行检测，通过2×2列联表得出EBA可检测到的抗体活性与健康状况之间的关系。在混合类型组中，66%临床患有衣原体病症状的鸟类抗体呈阳性，而临床患病的鸡尾鹦鹉中只有32.3%抗体呈阳性。使用卡方检验对列联表进行统计分析表明，EBA试验能够根据健康状况区分个体鸟类（P<0.001）。当通过EBA、LA和直接补体结合试验（DCF）检测配对的血清或血浆样本时，LA检测到的滴度显著降低（≥4倍变化）的比例最高，DCF检测到的滴度显著升高的比例最高。展示了配对和多个血清或血浆样本中EBA、LA和DCF滴度的示例，以显示可能出现的各种反应。结果反映了在对鸟类进行个体检测时所看到的变化，在第一个检测样本中观察到滴度存在变异性。概述了认为对于确认或排除鸟类感染过程必要的其他检测类型。给出了血清学结果的当前解释。
