Effects of osmotic stress on metabolism, shape, and amino acid content of Leishmania.

An acute decrease in osmolality causes a rapid change in the shape of the parasitic protozoan Leishmania donovani as determined by light microscopy and by flow cytometry. Incubation of the cells is an isotonic buffer supplemented with glucose. 2-deoxyglucose (2-DG), alanine, or proline also causes a shape change, presumably due to the swelling caused by the water that accompanies these substrates as they are actively transported into the cells. Hypo-osmolality also causes a rapid release of alanine and several other amino acids via a swelling activated amino acid channel. A sudden increase in osmolality causes a change in shape, an inhibition in the rates of oxidation of alanine, proline, leucine, and glucose, and in the rates of uptake of 2-aminoisobutyrate (AIB) and 2-DG. The protein kinase inhibitors staurosporine and genistein inhibited the rates of oxidation of alanine, glucose, and proline in a culture-age dependent manner and also altered the rate of release of AIB in response to hypo-osmotic stress. The possible roles of protein kinases in the culture-age dependent changes in the uptake, release and metabolism of several amino acids and of glucose are discussed.