Kozlov R S, Strachunskiĭ L S, Livermor D M, Stetsiuk O U, Shavrikova E P
London Hospital Medical College, UK.
Antibiot Khimioter. 1996;41(10):22-7.
To evaluate the adequacy of AGV agar for antimicrobial susceptibility testing, the susceptibility of a range of bacteria to 10 antimicrobials on AGV, Mueller-Hinton and isoSensitest agars, all supplemented with 5 per cent lyzed horse blood was determined. Disc tests were used. In general, AGV agar gave identical susceptibility results to Mueller-Hinton and isoSensitest agars for common gram positive and gram negative bacteria with most of the tested microbials excluding sulphonamides and trimethoprim. With those latter antimicrobials inhibition zones for susceptible organisms were not formed on AGV agar whereas large zones were present on Mueller-Hinton and isoSensitest agars. This discrepancy probably can be explained by the presence of high levels of thymidine in AGV agar; too high to be corrected even by the addition of 5 per cent lysed horse blood. AGV agar is possible to use for susceptibility testing with most of the microbials excluding trimethoprim and sulphonamides.
为评估AGV琼脂用于抗菌药物敏感性试验的适用性，测定了一系列细菌在添加5%溶血马血的AGV琼脂、Mueller-Hinton琼脂和isoSensitest琼脂上对10种抗菌药物的敏感性。采用纸片扩散法进行试验。总体而言，对于常见的革兰氏阳性菌和革兰氏阴性菌，在大多数测试抗菌药物（不包括磺胺类和甲氧苄啶）方面，AGV琼脂给出的敏感性结果与Mueller-Hinton琼脂和isoSensitest琼脂相同。对于后两种抗菌药物，AGV琼脂上未形成敏感菌的抑菌圈，而Mueller-Hinton琼脂和isoSensitest琼脂上有较大的抑菌圈。这种差异可能可以通过AGV琼脂中胸腺嘧啶核苷含量过高来解释；即使添加5%溶血马血也无法纠正过高的含量。除甲氧苄啶和磺胺类外，AGV琼脂可用于大多数抗菌药物的敏感性试验。
