Hrubec T C, Robertson J L, Smith S A
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061, USA.
Am J Vet Res. 1997 Feb;58(2):131-5.
To investigate the effects of poor water quality on hematologic and biochemical analytes in hybrid striped bass.
Hybrid striped bass (reciprocal cross: female Morone chrysops x male M saxatilis) maintained in 2,000-L tanks with undergravel filters.
Fish were acclimated to high ammonia (0.15 mg/L) and nitrate (200 mg/L) concentrations for 6 weeks prior to sample collection. Hematologic and biochemical profiles were determined for these fish and for fish kept under normal conditions (control). Comparisons were made among the 3 water qualities and with reference intervals determined previously.
Significant differences in hematologic and biochemical analytes were observed between fish in the various groups; however, most of the values were within established reference intervals. All values from fish in the high ammonia concentration tank were either within the reference interval or not significantly different from control values. Fish from the high nitrate concentration tank had higher serum creatinine values and lower chloride values than did control fish, and both analytes were substantially outside the reference intervals.
High ammonia concentration of 0.15 mg/L did not affect any of the blood analytes measured. The hypercreatininemia and hypochloremia observed in fish from the 200 mg of nitrate/ml tank were considered to be pathologic changes associated with the high nitrate concentration.
Determining the effects of water quality on hematologic and biochemical values helps to develop clinical pathology as a diagnostic tool in fish.
研究水质不佳对杂交条纹鲈血液学和生化分析物的影响。
杂交条纹鲈（正反交：雌性金眼狼鲈×雄性条纹鲈）饲养于配备底部过滤器的2000升水箱中。
在采集样本前，将鱼在高氨（0.15毫克/升）和硝酸盐（200毫克/升）浓度环境中驯化6周。对这些鱼以及处于正常条件下饲养的鱼（对照组）进行血液学和生化分析。比较了3种水质条件下的各项指标，并与先前确定的参考区间进行对比。
各实验组鱼的血液学和生化分析物存在显著差异；然而，大多数数值仍在既定的参考区间内。高氨浓度水箱中鱼的所有数值均在参考区间内，或与对照值无显著差异。高硝酸盐浓度水箱中的鱼血清肌酐值高于对照鱼，氯化物值低于对照鱼，且这两种分析物均大幅超出参考区间。
0.15毫克/升的高氨浓度未影响所测的任何血液分析物。在硝酸盐浓度为200毫克/毫升水箱中的鱼所观察到的高肌酐血症和低氯血症被认为是与高硝酸盐浓度相关的病理变化。
确定水质对血液学和生化值的影响有助于将临床病理学发展成为鱼类诊断工具。
