Bourry A, Poutrel B
Institut National de la Recherche Agronomique, Laboratoire de Pathologie infectieuse et immunologie, Nouzilly, France.
J Dairy Sci. 1996 Dec;79(12):2189-95. doi: 10.3168/jds.S0022-0302(96)76595-5.
The kinetics of antibodies in serum and milk directed against proteins from Listeria monocytogenes were studied using 4 lactating cows after infection was experimentally induced in the udder with four strains of serotypes 4b or 1/2a. Antibodies (IgG and IgA) in samples of composite quarter milk and serum of the cow were measured by indirect ELISA. Microtiter plates were coated with proteins obtained from the culture supernatant of L. monocytogenes 4b. After challenge, an IgG response in serum and milk to listerial infections in the udder occurred for all cows, although the response varied among cows. In sera, the IgG titers reached a peak at 9 to 13 wk after challenge and remained elevated until 21 to 33 wk after challenge. In milk, the IgG titer increased significantly 3 wk after the challenge for all cows. A weak and nonpersistent increase in IgA antibodies also occurred. These results indicate that IMI by L. monocytogenes induced an increase of antibodies in milk, which could be detected with an ELISA test using our antigenic preparation. Therefore, this antigenic preparation could be used for the evaluation of a new method of diagnosis for bovine mastitis caused by L. monocytogenes.
利用4头泌乳奶牛，在实验性地用4种血清型4b或1/2a菌株诱导乳房感染后，研究了血清和乳汁中针对单核细胞增生李斯特菌蛋白质的抗体动力学。通过间接ELISA法测定奶牛混合乳区乳汁和血清样本中的抗体（IgG和IgA）。微量滴定板用从单核细胞增生李斯特菌4b培养上清液中获得的蛋白质包被。攻毒后，所有奶牛的血清和乳汁中均出现了针对乳房李斯特菌感染的IgG反应，尽管不同奶牛的反应有所不同。在血清中，IgG滴度在攻毒后9至13周达到峰值，并一直保持升高直至攻毒后21至33周。在乳汁中，所有奶牛在攻毒后3周IgG滴度显著升高。IgA抗体也出现了微弱且不持久的增加。这些结果表明，单核细胞增生李斯特菌引起的乳房内感染诱导了乳汁中抗体的增加，使用我们的抗原制剂通过ELISA试验可以检测到。因此，这种抗原制剂可用于评估一种针对单核细胞增生李斯特菌引起的牛乳腺炎的新诊断方法。
