Morisaki I, Akiyama Y M, Miyawaki Y N, Mori Y
Division of Dentistry for the Disabled, Osaka University Faculty of Dentistry, Japan.
J Periodontol. 1997 Jan;68(1):7-11. doi: 10.1902/jop.1997.68.1.7.
Cyclosporin a is a drug used to control rejection of organ transplantation and autoimmune diseases; however, it has also been implicated in gingival overgrowth. The present study investigates the relationship between severity of gingival overgrowth and blood cyclosporin A (CsA) levels in Fischer rats treated orally with CsA. Thirty-six 15-day-old male rats were divided into six experimental groups and given powdered rat chow containing 0, 50, 100, 200, 300, and 400 micrograms CsA/g diet ad libitum for 40 days. At the end of the 40-day treatment period, whole blood samples were collected from each rat for assessment of CsA levels. The rats were then sacrificed and the gingival sulcus depth (pseudopocket) around mandibular molars measured to estimate gingival overgrowth. The blood levels of CsA in rats increased with increasing amounts of CsA provided in their food. A 100% incidence in gingival overgrowth was induced in all the rats treated orally with CsA. The overgrowth was more severe in buccal than in lingual gingiva. A significantly positive correlation was found between gingival sulcus depth and the blood CsA level (rs = 0.914, P < 0.0001; Spearman's correlation coefficient by rank). On histological examination, the overgrown gingiva consisted of a thickened epithelial layer and an accumulation of subepithelial fibrous connective tissue components without marked distortion of their proportion.
环孢素A是一种用于控制器官移植排斥反应和自身免疫性疾病的药物；然而，它也与牙龈增生有关。本研究调查了口服环孢素A（CsA）的Fischer大鼠牙龈增生严重程度与血液中环孢素A（CsA）水平之间的关系。将36只15日龄雄性大鼠分为6个实验组，随意给予含0、50、100、200、300和400微克CsA/克饲料的大鼠粉状食物，持续40天。在40天治疗期结束时，从每只大鼠采集全血样本以评估CsA水平。然后处死大鼠，测量下颌磨牙周围的牙龈沟深度（假性牙周袋）以评估牙龈增生情况。大鼠血液中的CsA水平随着食物中CsA含量的增加而升高。所有口服CsA治疗的大鼠均出现100%的牙龈增生发生率。颊侧牙龈增生比舌侧更严重。牙龈沟深度与血液CsA水平之间存在显著正相关（rs = 0.914，P < 0.0001；Spearman等级相关系数）。组织学检查显示，增生的牙龈由增厚的上皮层和上皮下纤维结缔组织成分堆积组成，但其比例无明显改变。
