Saito S
Department of Environmental Medicine and Informatics, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
Res Commun Mol Pathol Pharmacol. 1996 Dec;94(3):259-64.
The question as to the increase in zinc content in metallothionein in response to copper injection was examined. Each rat was injected intraperitoneally once with 0.9% NaCl or copper (2, 4 or 6 mg copper/kg b.w.). After copper injection, 54.0-62.9% of the hepatic zinc contents were found in the cytosol. Although the zinc contents in the cytosol and livers of copper-injected rats were higher than those of control rats, they did not increase in response to copper injection. The distribution profiles of the hepatic cytosol of copper-injected rats on a Sephadex G-75 column showed that the amount of the increased zinc was attributable to the metallothionein and high molecular weight protein fractions. Although the zinc contents in hepatic metallothionein of copper-injected rats were higher than those of control rats, they did not increase in response to copper injection. These results indicate that zinc contents in liver and in metallothionein did not increase in response to copper injection.
研究了腹腔注射铜后金属硫蛋白中锌含量的增加情况。每只大鼠腹腔注射一次0.9%氯化钠或铜（2、4或6毫克铜/千克体重）。注射铜后，发现54.0 - 62.9%的肝脏锌含量存在于细胞溶质中。虽然注射铜的大鼠细胞溶质和肝脏中的锌含量高于对照大鼠，但它们并未因注射铜而增加。注射铜的大鼠肝脏细胞溶质在葡聚糖凝胶G - 75柱上的分布图谱表明，增加的锌量归因于金属硫蛋白和高分子量蛋白质组分。虽然注射铜的大鼠肝脏金属硫蛋白中的锌含量高于对照大鼠，但它们并未因注射铜而增加。这些结果表明，肝脏和金属硫蛋白中的锌含量并未因注射铜而增加。
