Suzuki F, Miyamoto S, Takita M, Oshita M, Watanabe Y, Kakizuka A, Narumiya S, Taniguchi T, Muramatsu I
Department of Pharmacology, Fukui Medical School, Japan.
Biochim Biophys Acta. 1997 Jan 14;1323(1):6-11. doi: 10.1016/s0005-2736(96)00229-5.
We have cloned a cDNA encoding rabbit alpha 1d-adrenoceptor from the rabbit liver cDNA library. The deduced amino-acid sequence of this clone encodes a protein of 576 amino acids that shows strong sequence homology to previously cloned human, rat and mouse alpha 1d-adrenoceptors. The pharmacological radioligand binding properties of this clone expressed in COS-7 cells were similar to those of rat alpha 1d-adrenoceptors. Competitive RT/PCR assays revealed wide tissue distribution of the alpha 1d-adrenoceptor mRNA in rabbit, especially abundant in vas deferens, aorta, prostate and cerebral cortex.
我们从兔肝cDNA文库中克隆了一个编码兔α1d -肾上腺素能受体的cDNA。该克隆推导的氨基酸序列编码一个由576个氨基酸组成的蛋白质，它与先前克隆的人、大鼠和小鼠α1d -肾上腺素能受体具有很强的序列同源性。在COS - 7细胞中表达的该克隆的药理学放射性配体结合特性与大鼠α1d -肾上腺素能受体相似。竞争性RT/PCR分析显示α1d -肾上腺素能受体mRNA在兔体内广泛分布，尤其在输精管、主动脉、前列腺和大脑皮层中含量丰富。
