重新检查颈动脉体的超微结构，特别关注细胞间膜附着情况。

Re-examination of the carotid body ultrastructure with special attention to intercellular membrane appositions.

作者信息

Kondo H, Iwasa H

机构信息

Department of Anatomy, School of Medicine, Tohoku University, Sendai, Japan.

出版信息

Adv Exp Med Biol. 1996;410:45-50. doi: 10.1007/978-1-4615-5891-0_4.

DOI:10.1007/978-1-4615-5891-0_4

PMID:9030274

Abstract

Ultrathin sections of the carotid body of adult rats, processed through freeze-substitution after aldehyde-prefixation, showed a substantial number of presumed gap junctions between two adjacent chief cells, between chief and sustentacular cells, and between chief cells and carotid nerve terminals. The junctions showed a very narrow intercellular space of 2 nm and ranged in length from 200 nm to 1 micron. They may form the morphological substrate for electrical coupling between cells, the occurrence of which has been demonstrated by electrophysiology. Further studies using freeze-fracture and immunocytochemistry for connexins are necessary to confirm this possibility. In addition, small tight junctions are present between chief and sustentacular cells, and between adjacent sustentacular cells.

摘要

成年大鼠颈动脉体的超薄切片，经醛类固定后进行冷冻置换处理，结果显示，在相邻的两个主细胞之间、主细胞与支持细胞之间以及主细胞与颈动脉神经末梢之间存在大量推测为缝隙连接的结构。这些连接显示出非常狭窄的2纳米细胞间隙，长度范围从200纳米到1微米。它们可能构成细胞间电偶联的形态学基础，电生理学研究已证实细胞间存在电偶联。需要进一步采用冷冻断裂和连接蛋白免疫细胞化学方法进行研究，以证实这种可能性。此外，在主细胞与支持细胞之间以及相邻的支持细胞之间存在小的紧密连接。