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大鼠犁鼻神经上皮中的细胞间连接:冷冻断裂研究

Intercellular junctions in the rat vomeronasal neuroepithelium: a freeze-fracture study.

作者信息

Miragall F, Mendoza A S

出版信息

J Submicrosc Cytol. 1982 Oct;14(4):597-605.

PMID:7143514
Abstract

The intercellular junctions (tight junctions, desmosomes and gasp junctions) of the vomeronasal neuroepithelium of male Wistar rats were investigated by freeze-fracture. Tight junctions were localized apically sealing the intercellular clefts. Tight junctions were composed of 6 to 12 junctional strands arranged in a meshwork. the distance between the most apical and the most basal junctional strand was 430 +/- 90 nm. No proper distinction could be made between receptor-supporting and supporting-supporting cell tight junctions. Besides tight junctions, numerous desmosomes and ga junctions were found. Desmosomes were present on the supporting and receptor cell membranes below the level of the tight junctions. Gap junctions could be seen at the level of the perikarya of the supporting and receptor cells. They were only found on supporting cell membranes.

摘要

采用冷冻蚀刻技术对雄性Wistar大鼠犁鼻神经上皮的细胞间连接(紧密连接、桥粒和缝隙连接)进行了研究。紧密连接位于顶端,封闭细胞间隙。紧密连接由6至12条连接索组成,排列成网状。最顶端和最基部连接索之间的距离为430±90nm。在支持细胞与受体细胞间的紧密连接以及支持细胞间的紧密连接之间无法进行确切区分。除紧密连接外,还发现了大量桥粒和缝隙连接。桥粒存在于紧密连接水平以下的支持细胞膜和受体细胞膜上。缝隙连接可见于支持细胞和受体细胞的核周水平,且仅在支持细胞膜上发现。

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