Graziano V, Gerchman S E, Schneider D K, Ramakrishnan V
Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA.
Basic Life Sci. 1996;64:127-36. doi: 10.1007/978-1-4615-5847-7_13.
We have been engaged in studies of the structure and condensation of chromatin into the 30 nm filament using small-angle neutron scattering. We have also used deuterated histone H1 to determine its location in the chromatin 30 nm filament. Our studies indicate that chromatin condenses with increasing ionic strength to a limiting structure that has a mass per unit length of 6-7 nucleosomes/11 nm. They also show that the linker histone H1/H5 is located in the interior of the chromatin filament, in a position compatible with its binding to the inner face of the nucleosome. Analysis of the mass per unit length as a function of H5 stoichiometry suggests that 5-7 contiguous nucleosomes need to have H5 bound before a stable higher order structure can exist.
我们一直致力于利用小角中子散射研究染色质的结构及其凝聚成30纳米细丝的过程。我们还使用了氘代组蛋白H1来确定其在染色质30纳米细丝中的位置。我们的研究表明，随着离子强度的增加，染色质凝聚成一种极限结构，其单位长度质量为6 - 7个核小体/11纳米。研究还表明，连接组蛋白H1/H5位于染色质细丝内部，其位置与其与核小体内表面的结合相匹配。对单位长度质量作为H5化学计量比函数的分析表明，在稳定的高阶结构能够存在之前，需要有5 - 7个连续的核小体与H5结合。
