挥发性全身麻醉药对非洲爪蟾卵母细胞中表达的重组烟碱型乙酰胆碱α7、5-羟色胺3和嵌合型烟碱型乙酰胆碱α7-5-羟色胺3受体的作用。
Volatile general anaesthetic actions on recombinant nACh alpha 7, 5-HT3 and chimeric nACh alpha 7-5-HT3 receptors expressed in Xenopus oocytes.
作者信息
Zhang L, Oz M, Stewart R R, Peoples R W, Weight F F
机构信息
Laboratory of Molecular & Cellular Neurobiology, National Institute on Alcohol Abuse & Alcoholism, National Institutes of Health, Bethesda, MD 20892-8205, USA.
出版信息
Br J Pharmacol. 1997 Feb;120(3):353-5. doi: 10.1038/sj.bjp.0700934.
The effect of halothane and isoflurane was studied on the function of recombinant neurotransmitter receptors expressed in Xenopus oocytes. Both anaesthetics inhibited nicotinic acetylcholine type alpha 7 (nACh alpha 7) receptor-mediated responses, potentiated 5-hydroxytryptamine type 3 (5-HT3) receptor-mediated responses at low agonist concentrations, and inhibited the function of a chimeric receptor (with the N-terminal domain from the nACh alpha 7 receptor and the transmembrane and C-terminal domains from the 5-HT3 receptor) in a manner similar to that of the nACh alpha 7 receptor. Since the N-terminal domain of the chimeric receptor was from the nACh alpha 7 receptor, the observations suggest that the inhibition involves the N-terminal domain of the receptor.
研究了氟烷和异氟烷对非洲爪蟾卵母细胞中表达的重组神经递质受体功能的影响。两种麻醉剂均抑制烟碱型乙酰胆碱α7（nAChα7）受体介导的反应，在低激动剂浓度下增强5-羟色胺3型（5-HT3）受体介导的反应，并以类似于nAChα7受体的方式抑制嵌合受体（其N端结构域来自nAChα7受体，跨膜和C端结构域来自5-HT3受体）的功能。由于嵌合受体的N端结构域来自nAChα7受体，这些观察结果表明抑制作用涉及受体的N端结构域。
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