Lloyd R V, Jin L, Qian X, Kulig E
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
Am J Pathol. 1997 Feb;150(2):401-7.
The p27kip1 (p27) gene encodes an inhibitor of cyclin-dependent kinase activity. The expression of p27 protein in normal and neoplastic tissues was investigated by immunoblotting and immunohistochemistry. Immunoblotting studies detected a 27-kd protein band that was decreased in neoplastic pituitary tissues compared with normal pituitary. Immunostaining of 177 tissues showed abundant expression of p27 protein in normal tissues with decreased numbers of immunoreactive cells in adenomas and carcinomas in both endocrine and nonendocrine tissues. p27 expression was inversely related to the proliferation marker Ki-67 antigen detected with monoclonal antibody MIB-1. Parathyroid adenomas and hyperplasias had similar Ki-67 labeling indices; however, hyperplasias had threefold more p27-positive cells than parathyroid adenomas, suggesting that p27 immunostaining may be useful in distinguishing between these two conditions. These results indicate that there is widespread aberrant p27 expression in hyperplastic tissues and in benign and malignant neoplasms compared with normal tissues. Immunohistochemical analysis of p27 along with Ki-67 may be used to assess the biological behavior of various neoplasms, to classify hyperplastic and neoplastic tissues, and to study cell cycle regulation during tumor progression.
p27kip1（p27）基因编码一种细胞周期蛋白依赖性激酶活性抑制剂。通过免疫印迹和免疫组织化学方法研究了p27蛋白在正常组织和肿瘤组织中的表达情况。免疫印迹研究检测到一条27kd的蛋白条带，与正常垂体相比，肿瘤性垂体组织中的该条带减少。对177个组织进行免疫染色显示，p27蛋白在正常组织中大量表达，而在内分泌和非内分泌组织的腺瘤和癌中，免疫反应性细胞数量减少。p27表达与用单克隆抗体MIB-1检测的增殖标志物Ki-67抗原呈负相关。甲状旁腺腺瘤和增生的Ki-67标记指数相似；然而，增生组织中p27阳性细胞比甲状旁腺腺瘤多两倍，这表明p27免疫染色可能有助于区分这两种情况。这些结果表明，与正常组织相比，增生组织以及良性和恶性肿瘤中普遍存在p27表达异常。对p27与Ki-67进行免疫组织化学分析可用于评估各种肿瘤的生物学行为、对增生和肿瘤组织进行分类以及研究肿瘤进展过程中的细胞周期调控。
