Gazola R, Borella M I, Donaldson E M, Val-Sella M V, Sukumasavin N, Fava-de-Moraes F, Bernardino G
Escola de Farmácia e Odontologia de Alfenas, MG, Brasil.
Braz J Med Biol Res. 1996 May;29(5):659-64.
We report the plasma levels of estradiol-17 beta (E2), testosterone (T), 17 alpha-20 beta-dihydroxy-4-pregnen-3-one (17-20P), and cortisol (F) in female pacu during the reproductive cycle (N = 44) and in females induced to ovulate with an analogue of luteinizing hormone releasing hormone (LHRHa; 10 micrograms/kg) (N = 24). The plasma hormone levels were determined by validated radioimmunoassays. Females sampled during the reproductive cycle were grouped into 4 gonadal stages: resting, early maturation, advanced maturation and regression. The calculated gonadosomatic index varied from 0.5 +/- 0.1% in resting stage to 8.1 +/- 0.6% in advanced maturation stage. The E2 and T values were highest during the early maturation stage (E2 = 2172 +/- 7.1 pg/ml; T = 412 +/- 58 pg/ml) and the F values were highest during the advanced maturation stage (132 +/- 5 ng/ml). Females induced to ovulate by LHRHa injection were sampled at 0.6, and 12 h after injection of LHRHa. Two additional groups were sampled at ovulation and 24 h after ovulation. The E2 values were highest at 6 h (2917 +/- 65 pg/ml). The T and F values were highest at ovulation (T = 3498 +/- 77 pg/ml; F = 387 +/- 16 ng/ml) and 17-20P was detected only at ovulation (2163 +/- 80 pg/ml).
我们报告了雌性淡水白鲳在生殖周期（N = 44）以及用促黄体生成激素释放激素类似物（LHRHa；10微克/千克）诱导排卵的雌性（N = 24）体内雌二醇-17β（E2）、睾酮（T）、17α-20β-二羟基-4-孕烯-3-酮（17-20P）和皮质醇（F）的血浆水平。血浆激素水平通过经过验证的放射免疫测定法测定。在生殖周期采样的雌性被分为4个性腺阶段：静止期、早熟期、成熟后期和消退期。计算得出的性腺体指数从静止期的0.5±0.1%变化到成熟后期的8.1±0.6%。E2和T值在早熟期最高（E2 = 2172±7.1皮克/毫升；T = 412±58皮克/毫升），F值在成熟后期最高（132±5纳克/毫升）。通过注射LHRHa诱导排卵的雌性在注射LHRHa后0.6小时和12小时采样。另外两组在排卵时和排卵后24小时采样。E2值在6小时时最高（2917±65皮克/毫升）。T和F值在排卵时最高（T = 3498±77皮克/毫升；F = 387±16纳克/毫升），并且仅在排卵时检测到17-20P（2163±80皮克/毫升）。
