Stoclet J C, Andriantsitohaina R, L'heureux N, Martinez C, Germain L, Auger F
Laboratoire de Pharmacologie et de Physiopathologie Cellulaires URA CRNS 600, Université Louis Pasteur de Strasbourg, France.
Cell Biol Toxicol. 1996 Dec;12(4-6):223-5. doi: 10.1007/BF00438149.
Relatively limited information is available regarding the mechanisms controlling vasomotricity in human vessels. Isolated vessels obtained from patients undergoing surgery were used to characterize the role of endothelial factors and to study coupling mechanisms between receptors, intracellular calcium, and contraction. However, these investigations are limited by the availability of tissues and many uncontrolled factors. Cultured human vascular cells were also used, but these cells rapidly lose at least some of their differentiated characters. Recently, a human blood vessel equivalent was constructed in vitro from cultured cells, using tissue engineering. This technique allowed us to obtain vessel equivalents containing intima, media, and adventitia layers or tubular media layer only. Contraction and rises in intracellular calcium produced by agonists were studied, indicating that such human vessel equivalents may provide valuable models for pharmacological studies.
关于控制人体血管血管舒缩性的机制，目前可用的信息相对有限。从接受手术的患者身上获取的离体血管被用于确定内皮因子的作用，并研究受体、细胞内钙和收缩之间的偶联机制。然而，这些研究受到组织可用性和许多不受控制因素的限制。也使用了培养的人体血管细胞，但这些细胞会迅速丧失至少部分分化特征。最近，利用组织工程技术从培养细胞中体外构建了人体血管等效物。这项技术使我们能够获得包含内膜、中膜和外膜层或仅含管状中膜层的血管等效物。研究了激动剂引起的收缩和细胞内钙升高，表明这种人体血管等效物可能为药理学研究提供有价值的模型。
