Vandewalle A
Institut National de la Santé et de la Recherche Médicale, INSERM U246, Institut Fédératif de Recherche, Faculté de Médecine Xavier Bichat, Paris, France.
Cell Biol Toxicol. 1996 Dec;12(4-6):299-303. doi: 10.1007/BF00438161.
We summarize the results of study of the properties of two models of transimmortalized proximal tubule epithelial cells derived from the kidneys of transgenic mice harboring the SV40 large T and little t antigens/L-pyruvate kinase hybrid gene. The two cell lines, referred to as PKSV-PCT and PKSV-PR cells, maintained for long-term passages the main biochemical and functional properties from the convoluted and terminal parts of the proximal tubule, respectively, from which they were derived. In PKSV-PCT cells, gentamicin induced lysosomal alkalinization, decreased the cellular N-acetyl-beta-D-glucuronidase, and stimulated its secretion in a dose-dependent manner. The results indicate that these models of mouse proximal cultured cells could be suitable models for the study of the cellular action of drugs.
我们总结了对两种永生化近端肾小管上皮细胞模型特性的研究结果，这两种细胞模型源自携带SV40大T抗原和小t抗原/L-丙酮酸激酶杂交基因的转基因小鼠的肾脏。这两种细胞系分别称为PKSV-PCT和PKSV-PR细胞，在长期传代过程中分别保持了它们所源自的近端小管曲部和终末部的主要生化和功能特性。在PKSV-PCT细胞中，庆大霉素诱导溶酶体碱化，降低细胞内N-乙酰-β-D-葡萄糖醛酸酶，并以剂量依赖方式刺激其分泌。结果表明，这些小鼠近端培养细胞模型可能是研究药物细胞作用的合适模型。
