Castagnola M, Rossetti D V, Cassiano L, Misiti F, Pennacchietti L, Giardina B, Messana I
Istituto di Chimica Biologica, Università di Cagliari, Italy.
Electrophoresis. 1996 Dec;17(12):1925-30. doi: 10.1002/elps.1150171220.
Peptide electrophoretic mobility measured by capillary zone electrophoresis can be regarded as deriving from the mean of mobilities of different protonated forms, each one participating according to its charge. Stokes radius and relative percentage. The percentage is a function of the peptide dissociation constant and solution pH. Therefore, mobility modifications due to pH variations can be related to peptide dissociation constant, charge, and Stokes radius throughout general binding equations. Thus, not only can peptide dissociation constants be measured, but information about Stokes radius modifications linked to proton loss can also be obtained with picomoles of peptide.
通过毛细管区带电泳测量的肽电泳迁移率可被视为源自不同质子化形式迁移率的平均值，每种形式根据其电荷、斯托克斯半径和相对百分比参与其中。该百分比是肽解离常数和溶液pH的函数。因此，由于pH变化引起的迁移率变化可以通过一般的结合方程与肽解离常数、电荷和斯托克斯半径相关联。这样，不仅可以测量肽解离常数，而且用皮摩尔量的肽还可以获得与质子损失相关的斯托克斯半径变化的信息。
