Li J, Shimizu T, Miyoshi N, Yasuda N
Laboratory of Veterinary Pathology, Faculty of Agriculture, Kagoshima University, Japan.
J Vet Med Sci. 1997 Jan;59(1):17-22. doi: 10.1292/jvms.59.17.
Fusarenon-X (FX) 1.5 mg/kg was administered intraperitoneally (i.p.) to 6-week-old male Wistar rats for examination of pathologic effects on the glandular stomach. Rats ip-treated with sterilized physiological saline were used as control. FX-administered rats showed dilatation of the stomach with increased fluid contents after 1-4 hr. Light microscopically, a few apoptotic karyopyknosis were seen in chief cells in the basal region at 1 hr postadministration (PA) and mitotic inhibition was evident after 2 hr PA. Marked apoptosis of nuclear pyknosis and cytoplasmic inclusions in both zymogenic and oxyntic cells developed from basal to middle regions of the gastric mucous membrane at 2-4 hr PA with a peak at 3 hr. Apoptotic changes of differentiating neck cells and surface epithelia were less evident. Electron microscopy revealed that the chief cells were the main target of FX-induced apoptotosis. The parietal cells were secondarily involved because they phagocytosed chief cell-derived apoptotic bodies. In situ detection of DNA breaks by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) reaction revealed the positive nuclei after 1 hr PA, which increased with time and reached a peak at 3 hr PA, in accordance with apoptotic changes in histological study. Agarose gel electrophoresis of DNA isolated from the gastric mucosae of FX-administered rats showed ladder pattern of DNA fragments after 1.5 hr PA with the maximum distinctness at 3 hr PA.
将1.5毫克/千克的镰刀菌烯醇 - X（FX）腹腔注射给6周龄雄性Wistar大鼠，以检查其对腺胃的病理影响。用灭菌生理盐水腹腔注射处理的大鼠作为对照。注射FX的大鼠在1至4小时后出现胃扩张，胃内液体含量增加。光学显微镜下，给药后1小时，在基部区域的主细胞中可见少量凋亡性核固缩，给药后2小时有明显的有丝分裂抑制。给药后2至4小时，从胃黏膜基部到中部区域，酶原细胞和壁细胞中出现明显的核固缩凋亡和细胞质内含物，3小时达到峰值。分化的颈部细胞和表面上皮细胞的凋亡变化不太明显。电子显微镜显示，主细胞是FX诱导凋亡的主要靶点。壁细胞其次受累，因为它们吞噬了源自主细胞的凋亡小体。通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）反应原位检测DNA断裂，结果显示给药后1小时出现阳性细胞核，其随时间增加，在给药后3小时达到峰值，这与组织学研究中的凋亡变化一致。从注射FX的大鼠胃黏膜中分离的DNA进行琼脂糖凝胶电泳显示，给药后1.5小时出现DNA片段的梯形条带，在给药后3小时最为明显。
