Jeschke U, Briese V, Richter D, Kunkel S
Universität Rostock, Frauenklinik.
Z Geburtshilfe Neonatol. 1996 Sep-Oct;200(5):199-201.
Cytotrophoblast cells were isolated from human term placenta after cesarean section by fragmentation of villous tissue with trypsin and DNAse I. Trophoblast cells fuse in vitro to syncytiotrophoblast cells and progesteron is released. Placental Protein 14 (PP14) was incubated (300 micrograms/ml PP14 in 6 ml solution (6 x 10(6) cells) with the cytotrophoblast cells. Production of Progesteron is increased in PP14-trophoblast cell cultures compared to untreated trophoblast cells.
剖宫产术后，通过用胰蛋白酶和DNA酶I对绒毛组织进行破碎，从足月人胎盘中分离出细胞滋养层细胞。滋养层细胞在体外融合形成合体滋养层细胞并释放孕酮。将胎盘蛋白14（PP14）（6毫升溶液中含300微克/毫升PP14，6×10⁶个细胞）与细胞滋养层细胞一起孵育。与未处理的滋养层细胞相比，PP14 - 滋养层细胞培养物中孕酮的产量增加。
