Jenneckens I, Kriegesmann B, Brenig B, Jansen S
Tierärztlichen Institut, Universität Göttingen.
Dtsch Tierarztl Wochenschr. 1996 Oct;103(10):400-3.
Proacrosin is a molecule that is located under the acrosomal cap and that acts as a secondary ligand after acrosome reaction. Proacrosin is thought to bind to ZP2 (zona pellucida receptor 2). The high affinity is based on the interaction of the zona pellucida glycoprotein's acidic sulphate groups with basic, positively charged amino acids of the proacrosin molecule. Proacrosin is a serine protease and is capable to lyse the zona pellucida locally. Our structural analysis of proacrosin shows, that the catalytic centre is surrounded by loops containing positively charged amino acids which create the binding domain. This review is intended to describe the results that lead to the elucidation of the biochemistry of proacrosin.
前顶体素是一种位于顶体帽下方的分子，在顶体反应后作为二级配体发挥作用。前顶体素被认为与透明带蛋白2（ZP2）结合。这种高亲和力基于透明带糖蛋白的酸性硫酸基团与前顶体素分子中带正电荷的碱性氨基酸之间的相互作用。前顶体素是一种丝氨酸蛋白酶，能够局部溶解透明带。我们对前顶体素的结构分析表明，催化中心被含有带正电荷氨基酸的环所包围，这些环形成了结合域。本综述旨在描述那些有助于阐明前顶体素生物化学性质的研究结果。
