Stux S V, Ju S T, Nisonoff A
J Immunol Methods. 1977;17(1-2):39-46. doi: 10.1016/0022-1759(77)90074-6.
A method is described for the production in guinea pigs of large amounts of ascitic fluid containing non-specific IgG, antiprotein antibodies, complement, other serum proteins and leukocytes. The method is an adaptation of a procedure previously applied to mice. A major difference is the extended schedule of inoculations required for the induction of ascites in guinea pigs; a requirement for boosting with antigen intradermally while repeatedly inoculating adjuvant intraperitoneally; and the much larger quantities obtained. The average yield of ascitic fluid, when antigen was not used, was 113 ml per animal, and the average yield of IgG was 0.87 g. With antigen (keyhole limpet hemocyanin) the average yields were 143 ml and 1.6 g of antibody per guinea pig. Complement titers were 41 to 74% of those in serum. The number of leukocytes per ml of ascites ranged from 7 X 10(6) to 20 X 10(6). The method should be useful for the production of large amounts of leukocytes, antibodies and other serum proteins from a small colony of laboratory animals. In addition, cells can be obtained without the need to sacrifice the animal.
本文描述了一种在豚鼠体内产生大量腹水的方法，该腹水含有非特异性IgG、抗蛋白抗体、补体、其他血清蛋白和白细胞。该方法是对先前应用于小鼠的程序的一种改进。主要区别在于诱导豚鼠腹水所需的接种时间表延长；在反复腹腔注射佐剂的同时需要皮内注射抗原进行加强免疫；以及获得的产量要大得多。当不使用抗原时，每只动物腹水的平均产量为113毫升，IgG的平均产量为0.87克。使用抗原（钥孔戚血蓝蛋白）时，每只豚鼠的平均产量为143毫升和1.6克抗体。补体效价为血清中的41%至74%。每毫升腹水中白细胞的数量在7×10⁶至20×10⁶之间。该方法对于从一小群实验动物中大量生产白细胞、抗体和其他血清蛋白应该是有用的。此外，无需处死动物就能获得细胞。
