Yaron A, Dunham E K, Stashenko P P, Campos-Neto A, Levine H, Schlossman S F
J Immunol. 1977 Sep;119(3):968-75.
Strain 2 guinea pigs, immunized with Lys10-Lys(Dnp) in CFA and repeatedly injected intraperitoneally with adjuvant, developed ascites. The fluid was harvested over 8 months in total amounts up to 2 liters per animal and contained substantial amounts of cells and antibody which reacted with the immunizing antigen and related peptides. The antibody was of the IgG and IgA classes and showed restricted heterogeneity. Among synthetic Dnp-oligopeptides, both the cells, studied by antigen-stimulated thymidine incorporation, and the purified antibody, studied by fluorescence quenching, demonstrated the same specificity for the immunizing antigen as has previously been noted in lymph node cells and in serum antibody. The technique offers a means for studying more cells and more antibody than has previously been possible from individual guinea pigs.
用赖氨酸10-赖氨酸（二硝基苯基）在弗氏完全佐剂中免疫的2号品系豚鼠，经腹腔反复注射佐剂后出现腹水。在8个月的时间里，从每只动物身上总共收集到多达2升的腹水，其中含有大量与免疫抗原和相关肽发生反应的细胞和抗体。该抗体属于IgG和IgA类，显示出有限的异质性。在合成的二硝基苯基寡肽中，通过抗原刺激的胸腺嘧啶核苷掺入研究的细胞以及通过荧光猝灭研究的纯化抗体，对免疫抗原表现出与先前在淋巴结细胞和血清抗体中所观察到的相同特异性。该技术提供了一种手段，可用于研究比以往从单个豚鼠中所能获得的更多的细胞和更多的抗体。
