Caligo M A, Cipollini G, Berti A, Viacava P, Collecchi P, Bevilacqua G
Institute of Pathology, University of Pisa, Italy.
Int J Cancer. 1997 Feb 20;74(1):102-11. doi: 10.1002/(sici)1097-0215(19970220)74:1<102::aid-ijc18>3.0.co;2-h.
NM23 is a protein associated with tumor progression, expressed in all tissues and in human tumors. Reduced expression of NM23.H1 is related to high incidence of lymph node and distant metastasis or to poor prognosis of the patient in several human malignant tumors. In this study we analyze NM23 expression in non-neoplastic mammary tissues surrounding the tumoral lesions, in human mammary carcinomas and in lymph node metastasis. Our analysis shows that NM23.H1 expression is lower in the mammary cells surrounding the tumor than in the tumor itself. In the primary tumors we observed a negative trend between degree of local invasion and level of NM23.H1 expression. A further decrease of NM23.H1 was detected in the invasive tumors that metastasized to axillary lymph nodes and in the metastasis. NM23.H2 was always more highly expressed than NM23.H1, and reduced expression of NM23.H1 but not NM23.H2 was concordant with the presence of lymph node metastasis or local invasiveness of the primary tumor. A positive correlation between NM23.H1 mRNA content and cell growth rate of breast tumor cells has been confirmed. However, this trend was not maintained in cancer cells from tumors that metastasized to axillary lymph nodes and in metastatic cells; in these 2 situations the NM23.H1 mRNA content varied without any relationship to the proliferative rate of the cells. In addition, in comparison with the initial tumor, the metastatic cell population showed a strong decrease of NM23.H1 expression and increased proliferative activity.
NM23是一种与肿瘤进展相关的蛋白质，在所有组织和人类肿瘤中均有表达。NM23.H1表达降低与多种人类恶性肿瘤中淋巴结转移和远处转移的高发生率或患者的不良预后相关。在本研究中，我们分析了肿瘤病变周围的非肿瘤性乳腺组织、人类乳腺癌组织及淋巴结转移灶中NM23的表达情况。我们的分析表明，肿瘤周围乳腺细胞中NM23.H1的表达低于肿瘤本身。在原发性肿瘤中，我们观察到局部侵袭程度与NM23.H1表达水平之间呈负相关。在转移至腋窝淋巴结的浸润性肿瘤及转移灶中检测到NM23.H1进一步降低。NM23.H2的表达始终高于NM23.H1，NM23.H1而非NM23.H2表达降低与淋巴结转移或原发性肿瘤的局部侵袭性相关。已证实NM23.H1 mRNA含量与乳腺肿瘤细胞的生长速率呈正相关。然而，在转移至腋窝淋巴结的肿瘤的癌细胞及转移细胞中，这种趋势并未维持；在这两种情况下，NM23.H1 mRNA含量的变化与细胞增殖速率无关。此外，与原发肿瘤相比，转移细胞群体中NM23.H1表达显著降低，增殖活性增加。
