Motoyama T, Fujiwara M, Kojima N, Horiuchi H, Ohta A, Takagi M
Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Japan.
Mol Gen Genet. 1997 Jan 27;253(4):520-8. doi: 10.1007/s004380050353.
We previously isolated three chitin synthase genes (chsA, chsB, and chsC) from Aspergillus nidulans. In the present work, we describe the isolation and characterization of another chitin synthase gene, named chsD, from A. nidulans. Its deduced amino acid sequence shows 56.7% and 55.9% amino acid identity, respectively, with Cal1 of Saccharomyces cerevisiae and Chs3 of Candida albicans. Disruption of chsD caused no defect in cell growth or morphology during the asexual cycle and caused no decrease in chitin content in hyphae. However, double disruption of chsA and chsD caused a remarkable decrease in the efficiency of conidia formation, while double disruption of chsC and chsD caused no defect. Thus it appears that chsA and chsD serve redundant functions in conidia formation.
我们之前从构巢曲霉中分离出了三个几丁质合成酶基因（chsA、chsB和chsC）。在本研究中，我们描述了从构巢曲霉中分离并鉴定出的另一个几丁质合成酶基因chsD。其推导的氨基酸序列与酿酒酵母的Cal1和白色念珠菌的Chs3分别具有56.7%和55.9%的氨基酸同一性。chsD的缺失在无性繁殖周期中未导致细胞生长或形态缺陷，也未使菌丝中的几丁质含量降低。然而，chsA和chsD的双缺失导致分生孢子形成效率显著降低，而chsC和chsD的双缺失未导致缺陷。因此，chsA和chsD在分生孢子形成中似乎具有冗余功能。
