从小鼠肺cDNA文库中分离出小鼠MT2 - MMP基因并鉴定其产物。
Isolation of a mouse MT2-MMP gene from a lung cDNA library and identification of its product.
作者信息
Tanaka M, Sato H, Takino T, Iwata K, Inoue M, Seiki M
机构信息
Department of Molecular Virology and Oncology, Cancer Research Institute, Kanazawa University, Takara-machi, Ishikawa, Japan.
出版信息
FEBS Lett. 1997 Feb 3;402(2-3):219-22. doi: 10.1016/s0014-5793(96)01537-2.
We have isolated a new MT-MMP related gene of 3.3 kb from a mouse lung cDNA library using a human MT1-MMP cDNA as a probe. The deduced protein sequence shows 87% homology to human MT2-MMP and 52, 50 and 29% to MT1-MMP, MT3-MMP and MT4-MMP, respectively. Thus the gene is thought to be a mouse homologue of human MT2-MMP. A monoclonal antibody raised against a synthetic peptide recognized mouse MT2-MMP as a 70 kDa protein. Like MT1- and MT3-MMPs, mouse MT2-MMP caused activation of progelatinase A upon co-transfection into COS-1 cells.
我们以人MT1-MMP cDNA为探针，从小鼠肺cDNA文库中分离出一个3.3 kb的新的MT-MMP相关基因。推导的蛋白质序列与人MT2-MMP的同源性为87%，与MT1-MMP、MT3-MMP和MT4-MMP的同源性分别为52%、50%和29%。因此，该基因被认为是人类MT2-MMP的小鼠同源物。针对合成肽产生的单克隆抗体将小鼠MT2-MMP识别为一种70 kDa的蛋白质。与MT1-MMP和MT3-MMP一样，小鼠MT2-MMP共转染到COS-1细胞后可激活前胶原酶A。
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