van Gelder W, Cleton-Soeteman M I, Huijskes-Heins M I, van Run P R, van Eijk H G
Department of Chemical Pathology, Erasmus University, Rotterdam, The Netherlands.
Brain Res. 1997 Jan 23;746(1-2):105-16. doi: 10.1016/s0006-8993(96)01179-1.
The mechanism and regulation of iron transport to the brain are largely unknown. The large surface area of the blood-brain barrier capillaries and the presence of transferrin receptors on the luminal plasma membranes of the blood-brain barrier endothelial cells (BBB-ECs) suggest that these cells actively participate in the transport of iron into the brain. In this paper, we describe the ultrastructural morphology of primary and first-passage cultures of BBB-ECs grown on different types of porous membranes. To investigate the mechanism of iron transport into and across the BBB-ECs, porous membrane grown first-passage cells were incubated with 6.6-nm gold-labeled transferrin and studied with electron microscopy. Results are suggestive for a transcytosis of transferrin through the BBB-ECs.
铁向大脑转运的机制和调节在很大程度上尚不清楚。血脑屏障毛细血管的大表面积以及血脑屏障内皮细胞（BBB-ECs）管腔质膜上转铁蛋白受体的存在表明，这些细胞积极参与铁向大脑的转运。在本文中，我们描述了在不同类型多孔膜上生长的BBB-ECs原代培养物和首次传代培养物的超微结构形态。为了研究铁进入和穿过BBB-ECs的机制，将在多孔膜上生长的首次传代细胞与6.6纳米金标记的转铁蛋白一起孵育，并用电镜进行研究。结果提示转铁蛋白通过BBB-ECs进行转胞吞作用。
